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In a light microscope the resolution of the image it can project is limited by the distance each photon travels in its wavelength. Beneath this minimum distance, the "noise" of the photon's movement along its path overwhelms any resolution the light source may otherwise provide.
Light microscopes depend on light being reflected by the particles of the substance being studied. However, sometimes the particles are too small to noticeably reflect the light. Electron microscopes function by sending a beam of electrons through a subject; electrons are so tiny that anything can reflect them.
A scanning electron microscope would be the best choice because the electron microscope can achieve a much greater resolution than that obtained with the light microscope because the wavelength of electrons is shorter than that of light.
Resolving power of microscope is inversely related to the wavelength of the light used. So shorter the wavelength, greater the resolving power.
That depends on the microscope. A tunnelling microscope uses a "beam" of electrons as a detector - a charged ultra-sharp stylus tracks across a sample using the beam to sense distance to the sample and hence the micro-structure of the surface. In scanning microscopes, the image relies on the fact that electrons can be considered to be waves of very short wavelength. With decreasing wavelength, resolution is possible at increasing magnification - finer details are resolvable.
In a light microscope the resolution of the image it can project is limited by the distance each photon travels in its wavelength. Beneath this minimum distance, the "noise" of the photon's movement along its path overwhelms any resolution the light source may otherwise provide.
Changes in resolution with wavelength (light microscope) ... power improves as the wavelength of the illuminating light decreases. ...
The minimum resolvable separation distance of a light microscope depends on the wavelength of illumination and the numerical aperature. Because the electron beam has a far smaller wavelength than light used in light microscopy, it achieves far better resolution and it doesn't even involve the NE.
The transmission electron microscope operates on the same principle as the light microscope but uses electrons instead of light. What you can see with a light microscope is limited by the wavelength of light. Transmission electron microscopes use electrons as "light source" and their much lower wavelength makes it possible to get a resolution a thousand times better than with light microscope.
No the resolution would increase as wavelength of the illuminating light decreases.
The transmission electron microscope operates on the same principle as the light microscope but uses electrons instead of light. What you can see with a light microscope is limited by the wavelength of light. Transmission electron microscopes use electrons as "light source" and their much lower wavelength makes it possible to get a resolution a thousand times better than with light microscope.
Resolution of a microscope is tied to the numerical aperture of the objective lens and the condenser but is influenced by other factors, such as alignment, type of specimen, wavelength of light, and contrast enhancing techniques. Read more: Define Resolution in Microscopes | eHow.com http://www.ehow.com/facts_5753341_define-resolution-microscopes.html#ixzz1kYyrj6D9
1.operate by mean of light beam 2.the limitation of light microscope is depend on the resolution power 3.produce 2D image 4.magnification power is up to 1000x
Light microscopes depend on light being reflected by the particles of the substance being studied. However, sometimes the particles are too small to noticeably reflect the light. Electron microscopes function by sending a beam of electrons through a subject; electrons are so tiny that anything can reflect them.
A scanning electron microscope would be the best choice because the electron microscope can achieve a much greater resolution than that obtained with the light microscope because the wavelength of electrons is shorter than that of light.
Resolving power of microscope is inversely related to the wavelength of the light used. So shorter the wavelength, greater the resolving power.
That depends on the microscope. A tunnelling microscope uses a "beam" of electrons as a detector - a charged ultra-sharp stylus tracks across a sample using the beam to sense distance to the sample and hence the micro-structure of the surface. In scanning microscopes, the image relies on the fact that electrons can be considered to be waves of very short wavelength. With decreasing wavelength, resolution is possible at increasing magnification - finer details are resolvable.