Gram negative bacteria tend to be more?

Is the disease Lyme disease gram stain positive?

Spirochetes are not considered Gram positive or Gram negative. They tend to stain pink due to the last safrinin dye in the staining process, but its cell wall structure should make it Gram negative.

Is mycobacterium phlei gram positive or negative?

Although mycoplasma lack a cell wall and therefore test gram negative, they are considered to be descendents of 'nonsporulating and endospore forming gram-positive bacteria' (Madigan et al., 2009), such as Lactobacilli, Bacilli and Streptococci, (Dandekar et al., 2002), which have lost their cell wall. Mycoplasmas are therfore classed as gram- positive bacteria. This is supported by ribosomal RNA and DNA analysis.

Why is it necessary to use a young culture 24-hr old culture in gram staining?

less than 24 hours old. Older cultures tend to lose the ability to retain stains.

What does gram positive and gram negative mean?

Gram positive and gram negative is one way of classifying a bacteria.Gram positive bacterias have a cell wall which will stain in violet color. Gram negative bacterias, however, have an extra layer of cell wall around them which prevents the cell wall from being dyed. These gram negative bacterias will need an additional stain called safranin to make the bacterias redish, or pink color. Also Gram positive bacterias have a higher amount of RNA than gram negative baterias. This could be because indirectly peptidoglycan is a protein and the higher amount of protein production means a higher amount of RNA production.there is only difference in gram positive and negative is of cell wall as :The Gram positive cell wallThe Gram positive cell wall is characterised by the presence of a very thick peptidoglycan layer, which is responsible for the retention of the crystal violet dyes during the Gram staining procedure. It is found exclusively in organisms belonging to the Actinobacteria (or high %G+C Gram positive organisms) and the Firmicutes (or low %G+C Gram positive organisms). Bacteria within the Deinococcus-Thermus group may also exhibit Gram positive staining behaviour but contain some cell wall structures typical of Gram negative organisms. Imbedded in the Gram positive cell wall are polyalcohols called teichoic acids, some of which are lipid-linked to form lipoteichoic acids. Because lipoteichoic acids are covalently linked to lipids within the cytoplasmic membrane they are responsible for linking the peptidoglycan to the cytoplasmic membrane. Teichoic acids give the Gram positive cell wall an overall negative charge due to the presence of phosphodiester bonds between teichoic acid monomers.The Gram negative cell wallUnlike the Gram positive cell wall, the Gram negative cell wall contains a thin peptidoglycan layer adjacent to the cytoplasmic membrane, which is responsible for the cell wall's inability to retain the crystal violet stain upon decolourisation with ethanol during Gram staining. In addition to the peptidoglycan layer the Gram negative cell wall also contains an additional outer membrane composed by phospholipids and lipopolysaccharides which face into the external environment. The highly charged nature of lipopolysaccharides confer an overall negative charge to the Gram negative cell wall. The chemical structure of the outer membrane lipopolysaccharides is often unique to specific bacterial strains (i.e. sub-species) and is responsible for many of the antigenic properties of these strains.As a phospholipid bilayer, the lipid portion of the outer membrane is largely impermeable to all charged molecules. However, channels called porins are present in the outer membrane that allow for passive transport of many ions, sugars and amino acids across the outer membrane. These molecules are therefore present in the periplasm, the region between the plasma membrane and outer membrane. The periplasm contains the peptidoglycan layer and many proteins responsible for substrate binding or hydrolysis and reception of extracellular signals. The periplasm is thought to exist as a gel-like state rather than a liquid due to the high concentration of proteins and peptidoglycan found within it. Because of its location between the cytoplasmic and outer membranes, signals received and substrates bound are available to be transported across the cytoplasmic membrane using transport and signalling proteins imbedded there.

What is straphylococci?

Streptococcus is a Gram-positive bacteria which occur in chains. The term streptos in greek means twisted chains. Hence the name streptococcus. It causes bacterial pneumonia, endocarditis, meningitis etc Staphylococcus is a gram positive bacteria appearing round(coccus) and they form grape-like clusters. Most of them are harmless and appear normally on skin and mucous membrane of humans and other organisms.

Why are cats much more negative than dogs who are like always happy?

They are not really more negative, it is just the way you interpret their behavior. Cats tend to be more independent and passive, and people often read negative things into that.

What effect does the age of a culture have on its gram stain?

Yes, the age of the culture may influence the results of the stain. With the current theory behind gram staining, it is thought that in gram-positive bacteria, the crystal violet and iodine combine to form a larger molecule that precipitates out within the cell. The alcohol/acetone mixture then causes dehydration of the multilayered peptidoglycan in the gram-positive call wall, thus decreasing the space between the molecules and causing the cell wall to trap the crystal violet-iodine complex within the cell. In the case of gram-negative bacteria, the alcohol/acetone mixture, being a lipid solvent, dissolves the outer membrane of the gram-negative cell wall (and may also damage the cytoplasmic membrane to which the peptidoglycan is attached). The thin layer of peptidoglycan is unable to retain the crystal violet-iodine complex and the cell is decolorized. It is important to note that gram-positivity (the ability to retain the purple crystal violet-iodine complex) is not an all-or-nothing phenomenon but a matter of degree. There are several factors that could result in a gram-positive organism staining gram-negatively: 1. The method and techniques used. Overheating during heat fixation, over decolorization with alcohol, and even too much washing with water between steps may result in gram-positive bacteria losing the crystal violet-iodine complex. 2. The age of the culture. Cultures more than 24 hours old may lose their ability to retain the crystal violet-iodine complex. 3. The organism itself. Some gram-positive bacteria are more able to retain the crystal violet-iodine complex than others.

Do clean or dirty areas have more bacteria?

Dirty areas tend to have more bacteria than clean areas. Bacteria thrive in environments with organic matter and moisture, which are more commonly found in dirty areas. Regular cleaning and disinfection can help reduce bacteria levels in both clean and dirty areas.

Which elements tend to attract electrons to become negative ions?

Nonmetals tend to attract electrons to become negative ions.

Does Metabolism of fats produce more ATP molecules per gram than metabolism of carbohydrates or proteins?

When it comes to carbohydrates or fats producing more ATP, fats tend to produce more ATP per gram. Fats contain about 33 percent more ATP than carbs.

Supervisory responsibilities tend to bring out a person's negative side?

Supervisory responsibilities tend to bring out a person's negative side. true or false?

Does something have to be dirty to have bacteria on it?

No, bacteria can be present on surfaces that appear clean. Bacteria can be found virtually everywhere, including on surfaces that may not look dirty. Regular cleaning and disinfecting can help reduce the presence of bacteria on surfaces.