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What is CTAb?
CTAB is a buffer used in genetics to lyse (break down) the cell wall and membrane systems in a plant cell to allow DNA out of the nucleus so it can be studied. D-Train
What is the function of CTAB?
C-TAB is a detergent that helps lyse the cell membrane, however it is pretty poor with denaturing proteins so something with a longer tail is usually used for extraction.
What is the difference between CTAB and SDS method?
This refers to the type of detergent used to lyse cell membranes when extracting DNA from cells. SDS=Sodium dodecyl sulfate, CTAB=Cetyl trimethylammonium bromide
Why use CTAB in DNA isolation?
The CTAB extraction procedure is from Rogers and Bendich (1986). The magic bullet is supposed to be the separation of polysaccharides from nucleic acids by the use of CTAB. The technique capitalizes on the previous observations that nucleic acids can be selectively precipitated with CTAB. RNA and DNA are soluble in CTAB and 0.7 M NaCl but precipitate when the salt is reduced below 0.4 M. However, many polysaccharides are insoluble over this salt range and are thus not solubilized. CTAB is NOT used to lyse membranes in this procedure.
Role of ctab in the lysis of cell membrane?
none
Why plant is incubated with CTAB buffer at 65 degree celsius?
plant cell lysis
What is the importance of ctab buffer?
importance of ctab buffer
What is lyse?
its the disintegration of a cell by disruption of the plasma membrane
What does lyse mean?
Lyse is a biological term that means to burst or cut a cell.
A plant cell placed in a hypotonic solution will not lyse because?
The cell wall prevents the plant cell from bursting.
What could happen to a cell if its turgor pressure got too high?
The cell could lyse or explode.
What might happen if too water moved into a cell?
It could lyse (break)
