0
column ----250mm 4.6mm L1
flow rate.--- 1ml/minute
wavelength-- 195nmInjection Volume 10ul
temperature ambent
Buffer Preprations Take 600ml distilled water adjust pH 2.5 with phosphric acid
take 500mg 1-pentane sulphonic sodium salt. add 100ml distilled watere after disolved of sodium salt
Mobile Phase Ratio.
Buffer 7:
acetronitrile 3 (Buffer 700ml:Acetronitrile 300ml)
What else can I help you with?
What does HPLC stand for?
HPLC stands for High Performance Liquid Chromatography. It is a technique used to separate and analyze components in a liquid mixture based on their interactions with a stationary phase.
How does an HPLC work to separate and analyze compounds in a sample?
High Performance Liquid Chromatography (HPLC) works by using a liquid solvent to separate compounds in a sample based on their interactions with a stationary phase. The sample is injected into a column where the compounds travel at different speeds, allowing for separation. Detection methods like UV spectroscopy or mass spectrometry are then used to analyze and quantify the separated compounds.
Why reproducibility is important in hplc?
Reproducibility in HPLC ensures that results can be consistently obtained when the experiment is repeated, leading to reliable data. It allows for verification of results by other researchers and ensures the accuracy and reliability of the method. Reproducibility is crucial for validating the robustness of the HPLC method and for ensuring that results are accurate and can be trusted.
What is difference bet GLC and HPLC?
GLC has a stationary liquid phase and gas moving phase HPLC had a stationary solid phase and liquid moving phase HPLC is done under high pressure. HPLC can be used for thermally unstable compounds as opposed to GLC HPLC can be used for polar or low volatile compounds as opposed to GLC
Does HPLC can read histamine and TVB-N?
Yes, HPLC can be used to analyze histamine and TVB-N (Total Volatile Basic Nitrogen) in food samples. HPLC is a sensitive and selective technique that can separate and quantify various compounds, including histamine and TVB-N, based on their chemical properties. Pre-column derivatization may be required for some compounds to enhance their detection sensitivity in HPLC analysis.
What is rs-hplc?
"RS-HPLC method" means "Related Substance HPLC Method".
What is the definition of method development by hplc?
Method development is a process amenable to continuous improvement
What are the guidelines for method development of hplc according to ich?
the same guidelines for method validation
Which are the top commercial uses for HPLC?
HPLC is used to analyze, identify, purify and quantify organic compounds.
How Analysis of sodium nitrate in sodium nitrite by PDSA method?
Type your answer here.. you can analysed nitrite & nitrate on HPLC with C-18 column and methanol : Ammin buffer (30:70) at 210-215 nm. Naredra singh tomar Jiwaji Univ.Gwalior(MP)
What does HPLC stand for?
HPLC stands for High Performance Liquid Chromatography. It is a technique used to separate and analyze components in a liquid mixture based on their interactions with a stationary phase.
How do you find the amount sodium citrate in a cough mixture using hplc?
To find the amount of sodium citrate in a cough mixture using high-performance liquid chromatography (HPLC), you would first create a calibration curve using known concentrations of sodium citrate. Then, you would run the cough mixture through the HPLC and compare the peak area or height of the sodium citrate in the sample to the calibration curve to determine the concentration.
How you can analyze pregabalin on HPLC with UV detector?
We can quantitatively analyse pregabalin on hplc with uv detector, wavelength will be 210 n.m. and mobile phase will be 5 % acetonitrile. standard & sample solution preparation should be in mobile phase.
How does an HPLC work to separate and analyze compounds in a sample?
High Performance Liquid Chromatography (HPLC) works by using a liquid solvent to separate compounds in a sample based on their interactions with a stationary phase. The sample is injected into a column where the compounds travel at different speeds, allowing for separation. Detection methods like UV spectroscopy or mass spectrometry are then used to analyze and quantify the separated compounds.
Why reproducibility is important in hplc?
Reproducibility in HPLC ensures that results can be consistently obtained when the experiment is repeated, leading to reliable data. It allows for verification of results by other researchers and ensures the accuracy and reliability of the method. Reproducibility is crucial for validating the robustness of the HPLC method and for ensuring that results are accurate and can be trusted.
What is difference bet GLC and HPLC?
GLC has a stationary liquid phase and gas moving phase HPLC had a stationary solid phase and liquid moving phase HPLC is done under high pressure. HPLC can be used for thermally unstable compounds as opposed to GLC HPLC can be used for polar or low volatile compounds as opposed to GLC
How you find out Pka value of organic compound for HPLC method develoment?
If you can't find it in the literature it can be determined experimentally by titration.
