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Hematoxylin is only the drastic substance that these solutions contain. For histology, the two most commonly used are Mayer's Hematoxylin and Harris' Hematoxylin. They both contain water, hematoxylin and various salts.
Preperation of slides and Leifson flagella stain?
To remove excess stain before mounting
it helps in staining many slides at a time, as when we put many slides on a rack we can pour dye simoultaneously on all the slides and it will also help in reducing loss of extra dye or stain.
Longer incubation of gram-stained slides causes some gram positive bacteria to become gram negative.
Staining rack is used to hold many glass slides at a time. By putting slides on a staining rack, you can pour dye simultaneously and it will help to reduce extra dye or stain.
Using stains on slides help show some details that may be unclear otherwise, especially in cells.
Diff-Quick is a type of stain used to quickly evaluate slides in clinical practice. It consists of three containers of stain (although the first one is actually the fixer, not a stain). A slide is dipped in each one for about 10 seconds and then rinsed.
A Coplin jar is used in laboratory settings to hold and process multiple microscope slides at the same time. It is commonly used for staining procedures, such as the Gram stain, where multiple slides need to be immersed in various staining reagents simultaneously.
Gram staining is used to identify whether a bacterium is gram positive or gram negative. Slides can be dried using filter paper or tissues. The technique is based on the reaction of stain that happens with the membrane of bacteria.
There is no Gram stain for the rabies virus - it does not pick up either the stain or the counter-stain and has no official Gram stain status like bacteria do. When scientists are looking at slides of brains to see if an animal was infected with rabies, they use a special immunofluorescent stain made of antibodies against the rabies virus linked to either a vividly colored pigment or a fluorescent pigment. If the rabies virus is present, the antibodies in the stain adhere to the viral particles and then the pigment becomes fixed to the tissue as well, allowing the pathologist to "see" the virus (actually just that the virus is present and approximately where it is at - the virus is too small to see with a standard light microscope).
The difference between Wright Stain and Giemsa Stain is the intensity of the stain. The Giemsa Stain provides a better stain intensity than the Giemsa stain.