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It depends on the enzyme that you want to test. If the enzyme uses or produces a compound that is detectable, and you know what compounds the enzyme needs to use/produce this, you can add a known amount of the substrate (the compound that is used) to the enzyme and measure the product (the compound that is produced) over time.

This approach only works if there is only one enzyme that can act on the compound you are measuring.

If you already have that enzyme in pure form you can make an antibody against it and do an Enzyme linked immunosorbent assay (ELISA) or a Western blot, which will give you a signal if the enzyme is present.

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Q: How can you know or test that there is enzyme in the plant?
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