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How can you make phosphate buffer saline solution with pH 8.3?

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2007-06-29 18:18:19
2007-06-29 18:18:19

See the Related Links for "Columbia.edu: Phosphate buffer automatic calculator" to the bottom for the answer.

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I don't know how to make the solution below. Low salt buffer: 10 mM phosphate buffer, 10 mM NaCl, pH 7.4. Could you tell me the method in detail?


Phosphate buffer pH 6.8 preparation protocol below Stock solutions: 0.2M dibasic sodium phosphate (1 liter) Na2HPO4*12H2O (MW=358.14) --------71.64gm + dH2O to make 1 liter (Solution X) 0.2M monobasic sodium phosphate (1 liter) NaH2PO4*H2O (MW=138.01) --------27.6gm + dH2O to make 1liter (Solution Y) Working buffer: 0.1M (1 liter) pH 6.8 245 ml solution X + 255 ml solution Y ( filled up to 1 liter with dH2O)


you can prepare 20nM sodium phosphate buffer pH7.0. calculate mass of enzyme and dilute in volume buffer to reach concentration you want. good luck


To make this buffer solution you must combine the solutions of K2HPO4 and KH2PO4. To adjust the pH to 7.4 use a meter and add parts of each solution until the proper pH is met.


For pH = 7,0: 756 mL disodium hydrogen phosphate (Na2HPO4) solution 0,1 M (14,2 g/L) + 244 mL hydrochloric acid (HCl) solution 0,1 M


See the Related Links for "Columbia.edu: Phosphate buffer automatic calculator" to the bottom for the answer.



1 M Sodium Phosphate Buffer Stock Solution (1 liter) Protocol # Solution A: Dissolve 138.0 g NaH2PO4?H2O in 1 liter dH2O (pH 7.0). # Solution B: Dissolve 142.0 g Na2HPO4 in 1 liter dH2O (pH 7.0). # Mix 423 ml Solution A with 577 ml Solution B. # Autoclave and store at room temperature.


For the preparation of a solution with the pH=7,00:Add 29,1 mL sodium hydroxide solution 0,1 M to 50 mL potassium dihydrogen phosphate solution 0,1 M.


To make a 9 percent saline solution, start by preparing a 100 percent salt solution. With a bottle of 100 percent salt water, take 9 percent and dilute with distilled water to make a 9 percent saline solution.



Yes you need to know what molarity of the phosphate buffer you need to make and what voluime, then you can use Henderson-Hasselbalch equation. or simply use the phophate buffer calculator http://home.fuse.net/clymer/buffers/phos2.html


10ml of 0.4M Citric acis solution 90 ml of 0.4M sodium phsophate dibasic solution 12.22g NaCl (tomake 150mM ionic solution) or 6.38g to make 100mM ionic strength. make up volume upto 2000ml with water. You should not need to pH this buffer . :)


dissolve the 12 g of crystals of sodium phosphate in water to make 1oo ml


there is a special website for these calculations.. so please try them http://www.columbia.edu/~scb2001/tools/phosphate/phosphate.html


Dissolve 2.71 gm of potassium dihydrogen phosphate in 100 ml distilled water. Adjust the pH 7.4 by using 0.02 M Sodium hydroxide. Make the final volume upto 1 liter. Label as 50mM phosphate buffer. 2.71 gm makes the buffer 20 mM, right? 6.8 gm makes the buffer 50 mM.


Make Solution A by dissolving 174.18g of K2HPO4 in 1L of dH2O. Make solution B by dissolving 136g of KH2PO4 in 1L of dH2O. now mix solution A and B and finally adjust pH of your buffer.


Vinegar (acetic acid) is a weak acid, so yes it can be used to make a buffer.


dude i cant tell you way to private you better know this all ready use your bean ok it helps you to learn


can I make sodium acetate buffer 0.2M Ph=5 whit sodium acetate buffer 3m Ph=5 solution?


You can create a ph 9 buffer solution by mixing together a slightly stronger acid and a base, as the acid will override the bases basic functions and great an acidic solution.


that question does not make sense... do you mean are the salts in a saline SOLUTION in suspension? No... they are in solution. If you tried to seperate the salt from the water you could not unless you bolied off the water/cooled it down


Most veterinarians purchase a commercial salt solution for fecal floats.


8oz warm wateo mixed with 1tsp of water.


Buffer solution is used in experiments to maintain shape and to allow flow of electricity. Buffers maintain equilibrium in the pH, therefore it makes sure that you will not make the solution of your experiment over acidic or basic.



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