How can you make phosphate buffer saline solution with pH 8.3?
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Phosphate buffer pH 6.8 preparation protocol below Stock solutions: 0.2M dibasic sodium phosphate (1 liter) Na2HPO4*12H2O (MW=358.14) --------71.64gm + dH2O to make 1 liter (Solution X) 0.2M monobasic sodium phosphate (1 liter) NaH2PO4*H2O (MW=138.01) --------27.6gm + dH2O to make 1liter (Solution Y) Working buffer: 0.1M (1 liter) pH 6.8 245 ml solution X + 255 ml solution Y ( filled up to 1 liter with dH2O)
Yes saline solution (sterile 0.9% salt water) can be injected into breasts to make them bigger. More accurately the saline "inflates" the breasts. Results can be spectacular but short lived, as the saline solution absorbs into the body after a few days. Anyone practicising this temporary method of breast enhancement needs to take great caution to ensure that both the saline and the drip tubes and needles are sterile. If not, a potentially life-threatening infection…
A buffer is supposed to keep the pH of a solution from fluctuating too much. It helps keep the pH more-or-less consistent. Whether it keeps the solution neutral, it doesn't have to be neutral. You can make a solution be whatever pH you want, but different solutions and pHs require different approaches.
How many grams of acid and grams of base is needed to prepare 250 ml of phosphate buffer of pH 7.12?
How many ounces of 20 percent saline solution must be added to 40 ounces of a 5 percent saline solution to make a 10 percent saline solution?
When a person becomes dehydrated due to the loss of fluids and solutes saline solution water salts is infused into the bloodstream by medical personnel Why is saline solution used instead of pu?
Find a buffer at a reasonable buffering range (a pH of 4 or 7 is typically used as a standard). The pKa of the buffer does not have to be exactly the number you are looking for, but it should be close. Next use the henderson-hasselbach equation to find the ratio of weak acid to conjugate weak base to use in solution to get your desired pH.
A one percent saline solution (roughly isotonic) can be made by adding 1 teaspoon of salt to a litre of drinking water. The salt should not contain added iodine or anti-caking agents. Which means that kosher or pickling salt are appropriate but table salt isn't. The saline should be heated to a luke warm temperature before use.
Try using this equation: (F.W. with unit g/mol)(Molarity with unit mol/L)(vol. needed with unit L) = amt. reagent needed with unit g After the potassium is measured and your solution is made (minus a small amount of water for pHing), check the pH. Adjust pH according. Que solution up to final volume.