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There is a DNA killing step in RNA isolation by the enzyme DNase I. This will make sure your preparation is free of DNA.

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RNA is accociated with?

RNA (Ribonucleic Acid)...this is the messenger of the DNA (Deoxyribonucleic Acid) The RNA carries directions from the DNA to the Ribosomes that tell the Ribosomes what protein to make and how to do it. RNA reads the blueprint (DNA)


What are Free Nucleotides?

Free Nucleotides are ones that exist in the form of a triphosphate or three phosphates. When it is combined in DNA, the nucleotide loses two phosphates and only one phosphate is included in the DNA.


What is the function of primers?

RNA primers are used to initiate the DNA replication at the template strand. DNA molecules require a free 3' OH, to which it could add the nucleotides. This free 3' OH is provided by the RNA primer. So prior to the synthesis of DNA a short fragment of RNA is synthesized that is later excised and filled with DNA molecules.


What is the Role of RNAase in plasmid preparation?

RNAase is used in plasmid preparation to degrade RNA contaminants present in the sample. This helps to ensure that the isolated plasmid DNA is free from RNA, which can interfere with downstream applications such as PCR or cloning. RNAase treatment is an important step to obtain high-quality plasmid DNA.


What is RNA polymerase and tell its functions?

RNA polymerase is an enzyme responsible for synthesizing RNA molecules from a DNA template during transcription. It catalyzes the formation of phosphodiester bonds between ribonucleotides to produce an RNA strand complementary to the DNA template. RNA polymerase plays a crucial role in gene expression by transcribing the genetic information encoded in DNA into functional RNA molecules such as messenger RNA, transfer RNA, and ribosomal RNA.


What enzyme transcribes DNA?

The enzyme that transcribes the DNA into RNA is called RNA polymerase.


What is the name of the nucleotides have no DNA and no RNA?

Nucleotides do not have DNA or RNA. DNA and RNA are composed of nucleotides.


What attaches free nucleotides to the growing DNA strand?

During DNA replication, DNA polymerase binds free DNA nucleotides to an unzipped DNA strand. During transcription, RNA polymerase binds free RNA nucleotides to the unzipped anti-sense DNA strand.


What is the substance in RNA but not DNA?

An uracil base is in RNA but not in DNA


What base does RNA have that DNA does not have?

RNA has the base uracil that DNA does not have.


Are DNA and RNA carbohydrates?

Yes, DNA and RNA have different sugar . DNA contains deoxyribose sugar whereas RNA consists of ribose sugar, which are completely different from each other.


What are the 30 differences between DNA and RNA?

Comparing DNA and RNA, some key differences include: DNA is double-stranded, while RNA is single-stranded; DNA contains deoxyribose sugar, RNA contains ribose sugar; DNA has thymine base, RNA has uracil base; DNA is found in the nucleus, RNA is found in the cytoplasm; DNA is stable, RNA is less stable; DNA is the genetic material, RNA is involved in protein synthesis. These are just a few of the many distinctions between DNA and RNA.