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Q: How can you use alugbati or malabar nightshade as a staining agent?
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What is the Structure of mycobacterium tuberculosis in carbol fushin staining?

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Why would you use an irrigation staining technique opposed to direct application of iodine?

An irrigation staining technique is used to increase the contrast of soft tissue structures on an imaging study. This is done by injecting a contrast agent into the tissue. The contrast agent is typically iodine-based. Iodine-based contrast agents can be irritating to the tissue and can cause inflammation. This can lead to increased pain and discomfort for the patient. Additionally, iodine-based contrast agents can cause allergic reactions in some patients. An irrigation staining technique can help to minimize these side effects by diluting the contrast agent and evenly distributing it throughout the tissue. This can help to improve the comfort of the patient and increase the safety of the procedure.


You used acid-alcohol as the decolorizing agent?

If you used acid-alcohol as the decolorizing agent in spore staining the cells of the bacillus are gram plus. If you use acid alcohol it will not decolorize gram plus cell wall as only gram negative are decolorized. It will not get a differentiation in color.


Which step is most crucial or most likely to cause poor results in the gram stain?

Gram staining is a type of differential staining in which two types of bacteria are differentiated on the basis of their cell wall either gram positive or gram negative although all the steps in gram staining are crucial, the most important step the most crucial step in the performance of the Gram staining procedure is the decolorization step which is the Acid-Alcohol (3% HCl and 95% Ethanol) and must be timed correctly; the crystal violet stain will be removed from both Gram-positive and negative cells if the decolorizing agent is left on too long (a matter of seconds).


Why is acid alcohol rather than ethyl alcohol used as a decolorizing agent in the acid fast staining experiment?

Alcohol is a term used for any O-H group that is attached to a carbon. Perhapes the alcohol is found in a Safranin stain. I hope I have this right but if alcohol was used as the decolorizing agent, it may wash out too much stain to get a good view in an oil emersion microscope.


What are the gram positive diplococci?

They are bacteria, their morphology is basically two cocci cells fused with one another. They test positive for the Gram staining test. An example of GPDC is the bacteria genus Streptococcus pneumoniae, the major causative agent for pneumonia; a pathological lung condition


Why is aceto-orcein used?

as a staining agent perhaps... as we know, cells are hard to see if there is no coloring(stain)


What are the different techniques used in Biology?

Gram staining: This is to determine if a bacterial cell is Gram positive or negative. This uses Crystal violet dye, Gram's iodine as a mordant, Ethyl Alcohol as a decolorization medium, and Safranin as a secondary dye. Spore staining: Primary dye is Malachite green, then slide is placed over boiling beaker, cooled, rinsed with water, then Safranin is used as a counter stain. This test is used to show whether a bacteria is a spore former. Acid fast staining: Primary dye is Carbolfuchsin, heated over beaker like the spore stain, acid alcohol is used as the decolorizing agent, and Methylyene Blue is used as a counter stain. This is used to show bacteria with acid-fast walls, which have a thick waxy lipid around them. These are the most commonly used staining techniques with Bacteria.


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