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Which step is most crucial or most likely to cause poor results in the gram stain?
Wiki User
∙ 13y ago
Gram staining is a type of differential staining in which two types of bacteria are differentiated on the basis of their cell wall either gram positive or gram negative although all the steps in gram staining are crucial, the most important step the most crucial step in the performance of the Gram staining procedure is the decolorization step which is the Acid-Alcohol (3% HCl and 95% Ethanol) and must be timed correctly; the crystal violet stain will be removed from both Gram-positive and negative cells if the decolorizing agent is left on too long (a matter of seconds).
Wiki User
∙ 13y ago
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What are four possible results of a gram stain?
The four possible results after Gram staining are Gram positive, Gram negative, Gram variable and Gram in-determinant.
What happens when you leave a stain on the bacterial smear for too long?
If the bacterial cell are under stained then they will loose the stain of dye when wash with alocohol or may be simply by water which then cause a problem in identifying the cell type as e.g. in case of gram staining if the cell do not stain properly with methylene blue then they will loose the stain when washed and will counter stain with saffranin so the gram +ve will show the gram -ve colour.
What does a negative result for a spore stain indicate about the organism?
A negative result for the spores stain indicate that the gram-negative organism is present. A positive result for a spore stain indicates that a gram positive organism is present.
What is the difference between Wright stain and Giemsa stain?
The difference between Wright Stain and Giemsa Stain is the intensity of the stain. The Giemsa Stain provides a better stain intensity than the Giemsa stain.
Is maneval's stain an acid stain?
it is a basic dye that will stain the cells. That makes it a positive stain.
Can you stain a cutting board being used for food?
Not if the stain is likely to taint the food.
Will isopropyl alcohol stain clothes?
Since isopropyl alcohol is colorless, it's not likely to "stain" clothes.However, if the clothes are not completely colorfast, isopropanol is a pretty good solvent, so it may cause the color to fade in the area touched by it. There's also a distant possibility the dye used could react with the isopropanol in such a way as to cause it to change color, but this isn't likely.
Does nail polish remover stain bathroom tiles?
Stain, no. Remove color, very likely.
How do you remove polyurethane from a stain door without removing the stain?
You don't. You will most likely have to restain after stripping.
What makes a certain stain remover better than the next?
Stain removers work by interacting with the material of the stain and dissolving it. For example, modern enzyme stain removers contain protein enzymes which actually "digest" the stain. You have to find the remover which is designed to act on whatever made your stain to get the best results.
Will a flair pen stain cause your clothes to ripp?
Flair pen will not cause clothes to rip. It is possible that scrubbing the stain too harshly damaged the fabric. If you treat the stain gently, there is no reason that the clothing should rip.
What is aldehydes in PAS stain?
Aldehydes are what create the color in any stain; especially the PAS stain. In reference, the aldehyde are the pigments that can cause permanent staining in a fabric.
Why is decolorizing the most crucial step?
It is possible to decolourise gram positive bacteria and thus get false results of all gram negative organisms. Similarly, it is possible to under-decolourise and get all gram positive organisms. Ideally controls of known organisms should be run with each stain.
What is white stain on rabbit cage?
most likely dry pee
What are some of the best laundry stain removers?
The best stain remover depends on the type of stain and the type of material you use. The safest stain remover is water, but if you have a protein stain hot water will make the stain worse. Most people agree that bleach is the strongest stain remover, but unless the garment is white or color-safe bleach will cause a worse stain.
Is it common for men to have semen stain in underwear?
If a man puts on underwear after recently ejaculating, then it is likely that such a stain will be left. If a man ejaculates while actually wearing underwear, this will also leave a stain.
How do you get stain off white terry cloth fabric?
The best stain removal method depends on what type of stain. Since terry cloth is used in bathrooms, the stain is likely a protein stain, so do not wash it in hot water until the stain is gone. Pretreat the stain, rinse it out, and soak the fabric in bleach. Then rinse it again and wash it as you normally would.
