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How dilution of sample is achieved with the major technique for isolation of bacterial colonies?
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In what ways do the macroscopic features of bacterial colonies differ from that of molds?
Bacteria looks more glossy, white or yellow Molds will have a fuzzy look to them
When was federation achieved in Australia?
Australia's federation occurred on 1 January 1901.Prior to 1901, Australia was made up of six self-governing colonies; New South Wales, Victoria, South Australia, Queensland, Western Australia and Tasmania. On 1 January 1901, federation of the colonies was achieved and the Commonwealth of Australia was proclaimed. Australia's first Governor-General, John Hope, made the proclamation at Centennial Park in Sydney. Australia's first Prime Minister was Edmund Barton.
How you differentiate between Transformed bacterial colonies and satellite colonies?
Re-streak the center of the 'star' colony (transformed surrounded by satellites) on a plate contains the antibiotic, typically ampicillin. The colonies in the tertiary streak will most likely be the transformants. If you want to be quite sure, pick a single colony from the tertiary streak and re-streak again on a plate containing the antibiotic.
Why were Canadians loyal to Britain?
Britain and France explored the area and had colonies. France gave most of its colonies in North America to Britain in 1763 after the Seven Years' War. Over the years since then, a constitution was approved and more separation has been achieved. They are still part of the British Commonwealth.
What did the Spain lose as a result of the Spanish American war?
It lost its remaining colonies in the Pacific and the Caribbean, notably the Philippines and Cuba. Almost all of the other Spanish dominions in Latin America had already achieved independence.
Why is spread plate and pour plate technique not commonly used for isolation?
It is. Have you taken Microbiology? It is the most widely used isolation technique. The disadvantages to this technique are 1. colonies of several species may present a similar appearance 2. Certain bacteria species won't grow in this environment 3. Difficulty in removing colonies. EMB is the technique that's not commonly used.
Do yeast colonies resemble bacterial or fungal colonies?
bacterial
What is the technique you used in purify the bacterial culture on a plate?
Selective media, combined with quadrant streaking to dilute the sample into individual colonies.
Why is the production of an isolation streak key to the identifcation of a bacterium grown in culture?
Isolation streaking was first introduced by Robert Koch as an improvement on the dilution method of isolation of bacterial cultures. Isolation is achieved by taking a minute sample from within one bacterial colony and putting it in a sterile medium so that only that organism will grow. The assumption here is that a bacterial colony has ways of ensuring that no other bacteria will grow within its colonies, primarily by the use of antibiotic compounds that it produces and secondarily by the fact that it will consume available medium before any other organisms can establish themselves. Contamination of a sample by bacteria other than the one desired can lead to improper identification and poor test results. Good isolation such as that provided by the isolation streak method addresses this concern, is cost-effective, and produces acceptable results.
What is isolation of colonies?
not sure lool
Yeast colonies larger than bacterial colonies?
Not necessarily, some Bacillus can have big colonies
What is the purpose of the pour-plate technique?
used to assay bacterial contamination on food.
What is the use of resuspension solution in DNA isolation?
Resuspension buffer (solution I) is used for the isolation of plasmid DNA by alkaline lysis method. Bacterial cells, obtained from the culture (liquid culture or colonies grown on agar plate), is resuspended in this buffer. The purpose of this buffer is to provide an optimal starting pH (pH 8.0) and an ideal condition for subsequent lysis.
Why aren't molds streaked for isolation like bacteria?
Bacteria are streaked for isolation because they are UNICELLULAR and individual cells can be separated through streaking (creating colonies that arise from one single bacterial cell). Molds are MULTIcelluar and cannot be isolated; the cells need each other to form a mold. Single celled/unicellular fungi are called yeasts.
How many bacterial colonies are in a guinea pig mouth?
a lot
Do all bacterial colonies consist of pure cultures?
YES.!!
How to determine Bacterial load?
By pour plate and then counting the colonies.
