How do you locate genes on the chromosomes of humans?

Answer 1

Traditionally, radiolabeled oligonucleotides were used to locate genes on chromosomes. In this method, oligonucleotide sequences that are complimentary to a section of the gene being located are labelled with a radioactive isotope - P32. Following annealing, the gene location is detected through auto-radiography.

These days, fluorescent labels are used. These labels are more versatile and easy to detect. They also overcome the risks of using radioactivity.

Answer 2

Topcat Says: Fluorescent in situ hybridization is a technique used to identify the location of a gene or a specific DNA sequence on a chromosome. The technique involves hybridizing single-stranded DNA probes, labeled with a fluorescent dye, to their complementary DNA strand in the genome. Their location on the chromosome can be viewed under a fluorescent microscope.

Answer 3

I'm not sure how the mapping process is done, but in order to find a locus on a chromosome map, geneticists analyze the recombination data.