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Why is MgCl2 a variable in PCR?
Mg2+ complexes with the single stranded DNA that is to be amplified, and becomes the substrate of DNA polymerase. In other words, it helps in the binding of primer (and the subsequent target DNA) to the template DNA. Different volume of Mg2+ exert different complex-forming capabilities, and thus affects the end product of PCR.
Who were the other researchers to find the structure of DNA?
Luise
Who uses DNA?
Researchers who are doing life sciences!
Which explains how a probe can find a single-standard target DNA gene?
The probe is the second strand of DNA that forms double-stranded DNA with the target gene.
How Pulsed-Field Gel Electrophoresis differences from PCR identification of bacterial strain?
In using PCR to identify a bacterial strain, a single segment of DNA is amplified, typically an rRNA gene or a portion of it. The amplified fragment is then sequenced and the sequence compared to that of sequences in the databases. Since rRNA gene sequences are so conserved, this method does not have the resolution to distinguish individual strains of the same species, although it can be used for species identification. In PFGE, there is no amplification. Instead, restriction enzymes are used to digest chromosomal DNA to generate a characteristic pattern. Also, the detection method is staining of the DNA, not DNA sequencing
What controls which DNA is amplified in PCR?
The choice of primers controls which DNA is amplified in PCR.
Why is MgCl2 a variable in PCR?
Mg2+ complexes with the single stranded DNA that is to be amplified, and becomes the substrate of DNA polymerase. In other words, it helps in the binding of primer (and the subsequent target DNA) to the template DNA. Different volume of Mg2+ exert different complex-forming capabilities, and thus affects the end product of PCR.
Who were the researchers racing to find the structure DNA?
Luise
Who were the researchers racing to find the structure of DNA?
Luise
Who were the other researchers to find the structure of DNA?
Luise
Who uses DNA?
Researchers who are doing life sciences!
Role of additives in PCR?
Reactants: (dNTPs, template DNA (to be amplified), primers(bind to DNA to begin elongation of strand), DNA Polymerase (elongate DNA), & MgCl2) in buffer + H2O
What is a portion of DNA that codes for a trait known as?
The portion of DNA that codes for a functional product is known as a gene.
Which explains how a probe can find a single-standard target DNA gene?
The probe is the second strand of DNA that forms double-stranded DNA with the target gene.
How Pulsed-Field Gel Electrophoresis differences from PCR identification of bacterial strain?
In using PCR to identify a bacterial strain, a single segment of DNA is amplified, typically an rRNA gene or a portion of it. The amplified fragment is then sequenced and the sequence compared to that of sequences in the databases. Since rRNA gene sequences are so conserved, this method does not have the resolution to distinguish individual strains of the same species, although it can be used for species identification. In PFGE, there is no amplification. Instead, restriction enzymes are used to digest chromosomal DNA to generate a characteristic pattern. Also, the detection method is staining of the DNA, not DNA sequencing
What is the difference between the target cell DNA and source DNA?
DNA is deoxyribo nucleic acid chains. If the DNA is taken from a source such as a micro organism or a plant is known as source DNA. Targe cells are where your construct is going to be transfected, target cells posses their own target cell DNA.
Explain why the precise length target DNA sequence doesn't get amplified until the third cycle?
In the first polymerization step, a new DNA strand is synthesized onto each primer. Taq polymerase extends this new strand well beyond the target sequence, producing a new DNA strand that contains the downstream/upstream primer and is longer than the target. In the second cycle, the DNA is denatured and primers anneal to the original target DNA and to the new DNA strands produced in the first cycle. A mixture of small target sequences and larger hybrid DNA sequences are produced at the end of the second cycle. In the third cycle, the target DNA of defined length is reprimed with both upstream and downstream primers. Taq polymerase then synthesizes two copies of target DNA of defined length. The number of these defined target sequences is doubled with each subsequent round of PCR, thereby increasing logarithmically. Thus, a 40 cycle PCR would amplify a given template 240 , thus potentially producing 1.0 x 1012 DNA molecules. In the first polymerization step, a new DNA strand is synthesized onto each primer. Taq polymerase extends this new strand well beyond the target sequence, producing a new DNA strand that contains the downstream/upstream primer and is longer than the target. In the second cycle, the DNA is denatured and primers anneal to the original target DNA and to the new DNA strands produced in the first cycle. A mixture of small target sequences and larger hybrid DNA sequences are produced at the end of the second cycle. In the third cycle, the target DNA of defined length is reprimed with both upstream and downstream primers. Taq polymerase then synthesizes two copies of target DNA of defined length. The number of these defined target sequences is doubled with each subsequent round of PCR, thereby increasing logarithmically. Thus, a 40 cycle PCR would amplify a given template 240 , thus potentially producing 1.0 x 1012 DNA molecules.
