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Three methods are used mainly to cultivate viruses: 1ANIMAL INOCULATION: First used human volenteers for work on yellow virus fever. Land Steiner and Poper used monkey for isolation of polioviruses Most widely used animal is white mice. Animal inoculation have several disadvantages as Immunity may interfare with viral growth and the animal may harbour latent viruses. 2 EMBRYONATED EGGS: Method was developed by Burnet and virus can be inoculated in several routes as in chorio allantoic membrane, allontoic membrane, in to ammniotic cavity and also in yolk sac. 3 TISSUE CULTURE: The culture of whole organ or tissue fragment as well as dispersed cells on a nutreint medium is used. It can be done by three ways. A)ORGAN CULTURE: Embryonic organ or mall tissue fragments are cultured in vitro in such a manner that they retain their tissue architecture. B) EXPLANT CULTURE: Fragment of minced tissue can be grown on explant e.g. Adenoid tissue explant culture for adenovirus. C) CELL CULTURE: Dispersed or loosed cells are used in that method to grwo viruses . First washed with salt solution they dissociated in component cell by using protolytic enzyme such as trypsin and mechanical shaking. Presence of EDTA helps in disspersion of cells. It can be again divided in different parts. Primary culture Diploid culture and Contineous culture.

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15y ago
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13y ago

In the laboratory, three methods are commonly used for culturing animal viruses. These methods involve using living animals, embryonic eggs, or cell cultures.

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10y ago

virus can't be grown on culture media.

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Q: What are the methods of culturing viruses?
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