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What type of graph is used for acceleration?
Speed-Versus-Time, Distance-Versus-Time.
What is the purpose of wavelength scan?
Wavelength scans measure the absorbance and emission of light through a sample. Absorbance is proportional to concentration and a wavelength scan can be used to determine concentrations of a sample.
What is the difference between absorbance and transmittance?
Transmittance is the ratio of light energy that is falling on a body. Absorbance is how much light energy is actually going into the body.
Does the slope of a speed-versus-time graph represents acceleration?
exactly
In a plot of absorbance vs concentration what does the slope represent?
The slope of absorbance vs concentration reptresents the value of εb, where ε is the absorbtivity with units of (L/mol cm) and b is path length measured in cm.
Is there a simple equation to calculate the concentration given the absorbance and a time?
You need a graphic concentration versus absorbance.
How can you investigate absorbance versus time and not concentration versus time like you normally do for any kinetic investigation?
Too many words
Is wavelength or absorbance the dependent variable?
"absorbance"Since in the experiment, you probably choose the wavelength, then measure the absorbance (absorption?, the absorbance is the dependent variable.
What is the purpose of the blank?
Blank Sample in Spectrophotometry is used to measure the absorbance of light without sample. It is subtracted from the total absorbance for measurement of Absorbance from a sample's absorbance.
What is the purpose of blank samples?
Blank Sample in Spectrophotometry is used to measure the absorbance of light without sample. It is subtracted from the total absorbance for measurement of Absorbance from a sample's absorbance.
What is difference between specific absorbance and absorbance interms of spectroscopy?
specific absorbance- it is absorbance in a solution containing one gm of substance in 100 ml solvent in 1cm shell. so it is having a difference with absorbance which is negative logarithm of incident light to the transmitted light. divya.chakraborty@gmail.com
What is the reason for taking absorbance on y-axis and time on x-axis?
Convention holds that time be on the horizontal axis.
What is the different between primary absorbance and secondary absorbance in fluorescence?
in primary light absorbed by outer molecule while in secondary re-absorbance occurs
What is the relation of Beers Lambert's law in UV Visible spectrometer?
The Beer-Lambert law Absorbance = (extinction coefficent)(pathlength of light)(concentration) allows you to measure the absorbance of sample in a UV spec, and change the rate from absorbance units / time to change in concentration / time. the pathlength of light being the width of the cuvette and the extinctin coefficent being specific to the product molecule.
Why is absorbance plotted instead of percent transmission when following bacterial growth?
Because when we read absorbance, it's the amount of light absorbed by the bacteria itself. Absorbance is directly related to the amount of bacteria. More absorbance = more bacteria.
What type of graph is used for acceleration?
Speed-Versus-Time, Distance-Versus-Time.
What is the specific absorbance of aspirin?
If you have a spectrofotometer ( the thing to mesure the absorbance) then play with the setting and use a maximum. this will lay close to your specific absorbance or take the pharmacopea or a MERCK index
