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The simplest type of Punnet square is a 2x2 square. You split up the alleles for mother and father and place them on the sides of the large square. Then you "drop" them to determine the possible genotypic and phenotypic ratios. For example, if the mom is Aa and the dad is Aa as well, then using the square, the possible combinations will be AA, 2 Aa, and aa. In this case, assuming A is the dominant allele, then there will be a 3:1 ratio of dominant phenotypes to recessive phenotypes. The genotype ratio will be 1:2:1, indicating 1 homozygous dominant, 2 heterozygous and 1 homozygous recessive genotypes.

This can be done for sex linked traits too. Use XX and XY for the parents and make the same box. For example, if it is an X linked recessive disorder and it is between XY and XX' then the possible outcomes are XX, XX', XY and X'Y. Here there is a 1/2 chance that the child, regardless of gender will be normal, 1/4 that the child (girl) will be normal but a carrier and 1/4 that the child (boy) will be affected.

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Q: How do you make and interpret Punnet squares for Autosomal and sex-linked traits?
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