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keep casein in UV light for 40 mints
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Does Klebsiella pneumoniae digest casein?
P. vulgaris does digest casein. When placed on a casein agar plate, which is opaque, and incubated P. vulgaris will leave a "clear zone" where the casein is being broken down. This proves that protein hydrolysis occurs.
What is the most effective way to sterilize nutrient agar?
by autoclaving at 121°C for 15 minutes
Isolation media for actinomycetes?
the most recognized media for isolation of actinomycetes are starch casein and oat meal
Does water dissolve in casein?
Casein is not soluble in water.
How do you strelise blood agar?
You can't sterilize blood as it would hemolize. Blood is sterilized aseptically when it is purchased-meaning the animal (lets say a sheep) is killed in a certain way to ensure this happens. So the blood in your blood agar plate has already been aseptically sterilized
Why must use starch casein nitrate agar and what is the starch casein agar properties?
Because it is a selective medium
How do you sterilize starch casein agar medium?
Dear Friend You can autoclave this medium but be carefull about volume of media because in time of heating it overflows. I make about 300 cc medium in a 1L erlenmeyer.
Which ingredient in a phenylethyl alcohol agar supplies carbon and nitrogen?
pancreatic digest of casein
Does Klebsiella pneumoniae digest casein?
P. vulgaris does digest casein. When placed on a casein agar plate, which is opaque, and incubated P. vulgaris will leave a "clear zone" where the casein is being broken down. This proves that protein hydrolysis occurs.
What ingredient in PEA supplies nitrogen?
PEA agar, also referred to as Phenylethyl Alcohol Agar is a medium used by microbiologists to grow microorganisms. The ingredient that supplies nitrogen is casein.
What is the most effective way to sterilize nutrient agar?
by autoclaving at 121°C for 15 minutes
What is the nitrogen source in PEA agar?
Pancreatic digest of casein, papaic digest of soybean meal, beef extract, peptone
What happens to milk when the suspended proteins are hydrolyzed?
If the casein in the milk is hydrolyzed it will lose its opaqueness. Therefore, if the bacteria have the exoenzyme capable of casein hydrolysis, there will be a clear zone around the bacterial growth. If the organism lacks the exoenzyme to break down casein the skim milk agar will remain white and opaque.
Isolation media for actinomycetes?
the most recognized media for isolation of actinomycetes are starch casein and oat meal
Which ingredient in PEA supplies carbon?
Casein is the ingredient in PEA that supplies carbon, as well as nitrogen. PEA is also known as Phenylethyl Alcohol Blood Agar.
What are other names for casein?
αS1 casein αS2 casein β-casein κ-casein
Which ingredient supplies carbon in the mannitol salt agar?
mannitol is a type of sugar, so it supplies the carbon in the MSA medium
