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How do you tell if a substance contains protein by using biuret test?
Wiki User
∙ 11y ago
If Biuret Reagent is related to the presence of Protein macromolecules, then a positive result will change it's colour from blue to violet.
Wiki User
∙ 11y ago
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What color is a positive test for protein?
A positive test, when using Biuret reagent, would be purple-ish
What is used to test for the presence of proteins?
non
What color will a peptide bond be after using the Biuret test?
in biuret test the presence of peptide bonds gives a violet color. but it should be noted that a minimum of two peptide bonds should be present in the protein to get a positive answer to this test.
What is the advantages of using biuret reaction in place of measuring the absorption of the protein solution at 280 nm?
The absorption of proteins at 280nm is according to electrons from the rings on the amino acid such as His, Trp, etc. And if there is no such kind of amino acids in the protein, we might not be able to get what the amount of the protein really is. At the other hand, what makes biuret reaction work is by the copper ion reacting with the dipeptide bonds, since every amino acid has the peptide bond, it's more accurate and reasonable to use biuret reactions to determine what the amount of the protein is.
Seawater that contains a substance that fishes breathe?
Seawater and fresh water contains oxygen that fish absorb using their gill. So the substance is oxygen.
How does biuret solution test for protein?
Proteins are made of peptide bonds which is the target of reaction for biuret test. It's not truly specific for proteins; biuret itself also gives a positive result (hence the name of the test). However, proteins are fairly common and biuret (and similar peptide-containing compounds) are relatively rare.
What are proteins carbohydrates?
The carbohydrate contains solid elements called "Clint" and protein contains the pretty element called "Bailo".
When using Biuret reagent what indicates the presence of proteins?
Biuret solution turns a shade of purple if proteins are present. Specifically, it turns a shade of purple if peptide bonds are present. A peptide bond is the bond formed between amino acids.
Real life situation for using a protein test?
Two real life applications using protein testing (biuret Test) Inlcude: - Lab tecs testing for protein in urine samples. If protein is present in the sample this indicates there may be problems with the kidney or other organs You should google " Urine Protein Test " for a full answer - As well you could use this test to test for the presence of protein in the food you eat. Although many pre-packed foods already have this information it may come in handy. For more info on this test and how you could use it go on livestrong.ca or google " protein testing in food"
How do you identify a protein?
There is a special kind of test that can be used to identify whether proteins are present in any food or not. The presence of proteins in a food can be tested using Biuret solution, which is a mixture of sodium hydroxide solution and small amount of copper sulphate solution. A change to violet color will be observed if proteins are present in that food.
What is protein profiling?
protein profiling using 2d gel electrophorosis
How do you use the protein that you ingest?
you use the protein you ingest by using it for energy.
