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Is Nigrosin Acidic or basic?
acidic
Which of the following is an example of an acidic stain?
Basic dyes: Crystal violet, Methylene blue, Malachite green, Safranin. Acidic dyes: Nigrosin, Congo red
How are power work and time related?
power is required to do work and work efficiancy is the ammount of time it takes to do work.
Why does energy have the ability to do work?
Energy does not have the ability to do work, but we use energy to do work. Work is the application of force over a distance. The amount of energy changes how much work can be done, but energy technically does not do any work.
What is it called to work together?
team work
Can you stain a staphylococcus specimen with nigrosin?
Nigrosin is a dye made of synthetic ingredients. it is a negative stain, which is what is required to be able to stain staphylococcus.
Is Nigrosin Acidic or basic?
acidic
What color is a gram positive cell with nigrosin?
clear
Are nigrosin and malachite green a functional analogous pair of stain?
No
Is it possible to substitute methylene blue for nigrosin in the negative stain?
no
What is another example of a acidic dye besides Congo red?
Nigrosin
Why does nigrosin not stain bacterial cells?
Because Nigrosin is an acidic dye--carries a negative charge--and repels against the negatively charged cell walls of most bacteria.
Why cant methylene blue be used in place of nigrosin in negative staining?
Because negative staining requires the use of an acidic stain, which will not penetrate the cells because of the negative charge on the surface of the bacteria. As a result, the unstained cells can be easily identified against the colored background.
What are the materials used in capsule stain?
For the capsule stain Congo red or a Nigrosin solution can be used. Next, Maneval's stain is used.
What would the slide look like under the microscope if methylene blue is used instead of nigrosin?
The stain would stain the cells rather than the background
Describe the microscopic appearance of encapsulated streptococcus if stained with safranin?
Safranin (red) is used in gram staining and endospore staining as the secondary stain. Nigrosin is used in negative staining, staining only the background and not the bacteria. Therefore, the bacteria within the capsule would stain red from the safranin. (Like in endospore staining and negative gram staining, safranin would stain the bacteria red.) Nigrosin would stain the background of the organism just as it would in negative staining. Bacteria (within capsul): stained safranin red Capsule (outer layer of bacteria): clear Background of organism: stained dark with Nigrosin
Is counterstain a negative stain?
Yes. Eosin and acid fuchsin are also acidic dyes like nigrosin.
