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Spores are formed when cells are under unfavourable conditions, as for the bacteria they are means of survival. So the older the culture the higher the cell number in that culture, which means less nutrients for the cells. Under this conditions cells will start spore production. Depending on the "age" of the culture you can get a mixture of vegetative cells with spores inside and spores that are already released or mostly spores with rare vegetative cells, which means the cells are dead.
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Does heat affect stain when removing them eg. using hot water to remove a stain?
all answers are at http://www.youtube.com/hlkostaf96 and http://www.hlkostaf96.tk
Why did you use a young culture for flagella stain?
Young cultures are typically used for a flagella stain because they are healthier and livelier. This helps the flagella get seen easier since the bacteria are continuously moving.
What is the gram stain results for an L. lactus bacteria?
Lactococcus Lactus is a gram positive bacteria and therefore retains the darker staining and therefore shows on a gram stain as dark blue/violet colour. This is because the thick peptidoglycan cell wall retains the primary crystal violet stain.
How do surfactants work in stain removers?
Surfectants work in stain removers to remove the stain and not damage what the stain is on. It oxidizes the stain and lifts it out of whatever its in,
Does food dye stain?
Usually, it does stain.
How does culture age affect results of a spore stain?
Older cultures produce more spores. If the culture is not old enough, the spores will be few, and possibly undetectible.
What are the abnormal results of a sputum culture?
The presence of bacteria and white blood cells on the Gram stain and the isolation of a microorganism from culture, other than normal flora contamination, is evidence of a lower respiratory tract infection.
Did you get a pure culture in your subculture and how to know?
Ensuring that your culture is pure is very important to getting accurate results of your gram stain. One way to tell if your culture is pure is to test both the control and the experiment cultures. If you get a different result than the one you were expecting, you might have a corrupted culture. Making sure that you have several items of each is helpful, as well.
What makes a certain stain remover better than the next?
Stain removers work by interacting with the material of the stain and dissolving it. For example, modern enzyme stain removers contain protein enzymes which actually "digest" the stain. You have to find the remover which is designed to act on whatever made your stain to get the best results.
Where is steel used in Rusting out how acids affect the rate of corrosion?
stain
What would the results be if you used the TB decolorizer during the spore stain?
Depends if heat is used
Why did you use a young culture for flagella stain?
Young cultures are typically used for a flagella stain because they are healthier and livelier. This helps the flagella get seen easier since the bacteria are continuously moving.
How would the pH of the stain methylene blue affect the staining of bacteria?
the ph of the stain on the bacteria caused by methylene blue would not affect it a lot since all methylene blue is supposed to do is make it visible on the microscope for e.g.
Does heat affect stain when removing them eg. using hot water to remove a stain?
all answers are at http://www.youtube.com/hlkostaf96 and http://www.hlkostaf96.tk
What are four possible results of a gram stain?
The four possible results after Gram staining are Gram positive, Gram negative, Gram variable and Gram in-determinant.
What is the basic for gram stain results between different bacteria?
its the difference in cell wall of both the type of bacteria
Methylene blue can be prepared as a basic stain or an acidic stain. How would the pH affect the staining of bacteria?
# The pH will determine if the bacteria will have a particular charge. If the chromophore is a positive ion like the methylene blue in the equation shown in the reading, the stain is considered a basic stain; if it is a negative ion, it is an acidic stain. Most bacteria are stained when a basic stain permeates the cell wall and adheres by weak ionic bonds to the negative charges of the bacterial cell.
