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What would the results be if you used the TB decolorizer during the spore stain?
Wiki User
∙ 13y ago
Depends if heat is used
Wiki User
∙ 13y ago
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What does a negative result for a spore stain indicate about the organism?
A negative result for the spores stain indicate that the gram-negative organism is present. A positive result for a spore stain indicates that a gram positive organism is present.
What does a positive result for spore stain indicate?
The spore was neutralized before stain removal insued.
What mordant is used in spore staining?
Heat is the mordant used in the spore stain, it fixes the primary stain.
What color is moraxella catarrhalis after the decolorizer is used?
Moraxella catarrhalis is a Gram negative bacterium. Thus, after the decolorizer is used, its thin cell wall won't retain the primary stain. The stain is washed away, and Moraxella catarrhalis is colorless.
How do you make decolorizer for gram stain?
It is an alchoholic compound i.e. 95% ethanol.
Why is it necessary to perform a spore stain when you can see the presence of spores in simple stained cells?
spore is a dormant structure and necessary to stain because in this we use malachite green which will retain in spore
What color do bacterial endospores stain in the Schaeffer-Fulton spore stain?
Green
Why gram staining procedure cannot stain endospores?
Because stains can't penetrate the spore covering. You have to heat the spore to open it and let the stain in.
How does culture age affect results of a spore stain?
Older cultures produce more spores. If the culture is not old enough, the spores will be few, and possibly undetectible.
Why is it essential to apply heat during endospore staining?
because they cannot be differentiated from inclusions of stored material without a special stain .
What is the counterstain in a spore stain?
the counter stain is safranin 0.5%
What is the purpose of staining the smear in malachite green during spore staining?
the purpose of boiling of smear in malachite green is to forces a stain to penetrate the endospore wall, it is necessary to heat the slide and the stain to prod the wall to allow the stain to enter.
