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Because stains can't penetrate the spore covering. You have to heat the spore to open it and let the stain in.
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What staining procedure makes use of pararosanaline?
flagellar stain
Are endospores acid fast?
No, endospores are not acid fast because they are not cells. Endospores will stain with the acid fast stain and look fuchsia but they are not positive for acid fast.
What color do bacterial endospores stain in the Schaeffer-Fulton spore stain?
Green
Is carbol fuchsin the same as safranin?
No. safranin is the classic stain used in gram staining. Concentrated Carbol Fushin is mainly used for the ZN staining procedure to stain organisms such as Vibrio cholerae and Cryptosporidium. Diluted Carbol Fushin can however be used as a replacement counterstain for Safranin in the gram stain.
Do you apply waterproofing product before or after staining the deck?
After. If you waterproof before staining, the stain won't make it to the wood to stain it.
What dye is used to stain endospores?
To differentiate the spores from other similar looking structures in the cell that don't stain as the spores do.
What staining procedure makes use of pararosanaline?
flagellar stain
What color are endospores after endospore stain?
After gram staining an endospore the color it would show up would be colorless or clear. It will not work for endospores because of its tough outer layer, stains can't penetrate.
How do you know when to do a spore stain?
endospores can't be stained by ordinary methods, such as simple and gram staining, because the stain can't penetrate the endospore's wall, therefore you must heat the stain to help it penetrate the wall
What is the appearance of capsule after being stain with capsule stain procedure?
capsule does not gets stained it appears to be colourless when stained using manewals staining procedure
What color are bacterial endospores after a gram stain is performed?
Gram-staining does not stain the endospore due to the tough, resistant water-proof structure. It appears as an unstained area in a vegetative cell. Malachite green must be forced into the endospore with heat to stain it.
Are endospores acid fast?
No, endospores are not acid fast because they are not cells. Endospores will stain with the acid fast stain and look fuchsia but they are not positive for acid fast.
Why is it ot necessary to include a negative control for the spore staining procedures?
When you stain endospores, the control sample is the cell itself, since you are only looking at the spores, which are present inside the cell.
What color do bacterial endospores stain in the Schaeffer-Fulton spore stain?
Green
What is the specific diagnostic value of the staining procedure?
Each stain is specific for specifikc materials by example if you want to check for iron in a certain tissue and you did not stain with its specific stain you will be never able to finde it
What is a primary stain?
If your talking about Acid Fast staining (aka Ziehl-Neelsen staining), after the addition of the primary stain (carbol fuchsin) heat is applied in order to facilitate proper staining. Bacteria such as Mycobacterium contain large amounts of lipid substances within their cell wall called mycolic acids. These acids resist staining by ordinary methods such as gram staining (where heat is not applied after primary staining). On application of heat, the stain carbol fuschin penetrates the cell wall and stains the cells pink. Following the secondary staining (methylene blue) the acid fast positive cells appear pink while others are stained blue. Endospore staining is yet another staining technique where heat is applied after primary staining (malachite green). In this case the spores are impermeable to stains, hence heating favours proper permeation of stain. Endospores appear green while vegetative cells appear red (secondary stain saffranine). Not all staining procedures involve applying heat after primary staining. However, heat is applied before even beginning the staining procedure. This is called heat fixing, where the cells are fixed so that they are not washed away during the subsequent washing steps.
Is carbol fuchsin the same as safranin?
No. safranin is the classic stain used in gram staining. Concentrated Carbol Fushin is mainly used for the ZN staining procedure to stain organisms such as Vibrio cholerae and Cryptosporidium. Diluted Carbol Fushin can however be used as a replacement counterstain for Safranin in the gram stain.
