Doxorubicin inhibits methylation of DNA.
Doxorubicin has a solubility of 10mg in 1 ml of water, which should be warmed slightly.
Ethidium bromide interchalates with DNA. It doesn't affect electrophoresis, but it help visualise the DNA bands after electrophoresis. The EtBr that is bound to the DNA will fluoresce under ultraviolet light.
Cytosine bases may spontaneously change into uracil bases. DNA has an enzyme asssociated with it that corrects this fault. If DNA contained uracil as a base, the repair enzyme would have no way of distinguishing between uracils normally present in the DNA code and uracils that had formed from cytosine. Therefore thymine is present instead of uracil.
Primers with more GC content will affect the melting temperature. Higher GC content will have a higher melting temperature.
Dna has a hydrophilic and hydrophobic side, also you have to remember that DNA is a polar molecule
what is the rules of methylation in transduction
methylation of DNA prevents over expression of DNA and also protects it from harmful degradation from DNase enzymes.
Methylation protects DNA from nucleases, endonucleases
methylation of DNA
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Xiaolin Zhang has written: 'Mutagenic mechanisms associated with DNA cytosine methylation, DNA base sequence context and DNA precursor pool asymmetry' -- subject(s): Mutagenesis, DNA, Nucleotide sequence, Methylation
The methylation of DNA prevents the cleavage of its own DNA
The newly replicated DNA regions can be terminated by methylation chemical modification. Methylation is a methyl group alkylation of hydrogen atom replacement.
Epigenetic Phenomena.
Methylation
Very high transposition is danger for genom. Because, the function of genom bereaks down.Both transposons and genom itself don not want it. Therefore, Transpozsons replicate themselver before methylation of DNA. after DNA replication, DNA is methylated. replication of transpositin occurs until methylation of DNA
chemical modifications of histones and DNA methylation.