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Ethidium bromide interchalates with DNA. It doesn't affect electrophoresis, but it help visualise the DNA bands after electrophoresis. The EtBr that is bound to the DNA will fluoresce under ultraviolet light.
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What explains the role of gel electrophoresis in forensic science?
It is a special technique used to separate and identify DNA fragments.
Some viva voce questions for electrophoresis?
1. WHAT IS ELECTROPHORESIS AND WHAT ARE THE IMPORTANTAPPLICATIONS OF ELECTROPHORESIS?Ans. Movement of charged particle in the electric field either towards cathode or anode whensubjected to an electric current is called electrophoresis.The following factors influence the movement of particles during the electrophoresis.(a) Electric current.(b) Net charge of the particle.(c) Size and shape of the particle.(d) Type of supporting media.(e) Buffer solution.Important Applications of ElectrophoresisThe technique of electrophoresis is used to separate and identify the(i) Serum proteins(ii) Serum lipoproteins(iii) Blood hemoglobins2. WHAT ARE THE DIFFERENT TYPES OF ELECTROPHORESIS?Ans. (a) Moving boundary electrophoresis: This technique was first introduced by TISELIUS in 1937(b) Zone electrophoresis: In this type of electrophoresis different types of supporting mediaare used. These are;(a) Paper electrophoresis(i) Whatman filter paper(ii) Cellulose acetate(b) Gel electrophoresis(i) Agarose.(ii) Polyacrylamide gel (used for the separation of isoenzymes).(iii) SDS-PAGE.(iv) Iso-electric focussing (proteins seperated in a medium possessing a stable pH gradient).(v) Immuno electrophoresis (for the separation of immunoglobulins).
What is the function of ethidium bromide in DNA extraction?
EtBr interculates into DNA and when exposed to UV light, it causeses DNA to nick and therefore uncoil
What is the definition for paper electrophoresis?
it is a separation technique
What process uses electricity to seprate organic compounds?
electrophoresis=)
Why is destaining done after staining in agarose gel serum electrophoresis?
To remove extra Etbr and higher background fluorescence.
Is etbr transfer with DNA during southern blotting?
The answer is NO! To me, it makes no sense, why we have to transfer etbr with DNA to a blotting membrane? When Southern blotting signals will be detected by means of Radiochemicals or fluro labelling why we have to think about EtBr?
What is the role of formaldehyde and formamide in RNA gel electrophoresis?
Denatures the RNA
What is the function of EtBr?
Ethidium Bromide is used for visualising DNA. When EtBr binds DNA it will glow pink under UV light. This allows you to take a picture of DNA bands in a gel. The gel is soaked in an EtBr solution and then lit up by UV light. Alternatly the EtBr can be incorporated into the gel beforehand but gives a poorer picture.
What is the role enzymes play in the procces of gel electrophoresis?
Gel electrophoresis is an analytical method used for the separation of DNA, RNA or proteins based on size.Enzymes are not requires to carry out this process
What is used to sort DNA into different lenghts?
Gel Electrophoresis
What explains the role of gel electrophoresis in forensic science?
It is a special technique used to separate and identify DNA fragments.
What is role of agarose of electrophoresis?
agarose helps in the separation of DNA bands by controlling the pore size of agarose gel
Role of ethidiumbromide in gel electrophoresis?
it is a visualising agent which enables you to watch the seperated DNA under the u.v light
What has the author A J Houtsmuller written?
A. J. Houtsmuller has written: 'Agarose-gel-electrophoresis of lipoproteins' -- subject(s): Blood protein electrophoresis, Electrophoresis, Gel electrophoresis, Lipoproteins
When was Electrophoresis - journal - created?
Electrophoresis - journal - was created in 1980.
Why gel red can stain DNA fragments?
The DNA is a red color band under UV light on EtBr staining because EtBr intercalate and binds double stranded DNA unspecifically, when it absorbs UV light, it emits red color.
