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The answer is NO!
To me, it makes no sense, why we have to transfer etbr with DNA to a blotting membrane? When Southern blotting signals will be detected by means of Radiochemicals or fluro labelling why we have to think about EtBr?
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∙ 12y ago
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Why gel red can stain DNA fragments?
The DNA is a red color band under UV light on EtBr staining because EtBr intercalate and binds double stranded DNA unspecifically, when it absorbs UV light, it emits red color.
How do you get agarose gel to solidify when it doesn't solidify after 10 min in the refrigerator?
I usually add EtBr
Why is destaining done after staining in agarose gel serum electrophoresis?
To remove extra Etbr and higher background fluorescence.
Minimum DNA on agarose to visualize?
you need at least 20ng to visualize it on an agarose gel along with EtBr or GR safe
What is the function of TEB buffer in gel electrophoresis?
Restriction buffer maintains the pH in a range suitable for enzyme activity, as well as supplying salt cofactors required for catalysis. Since different restriction enzymes require varying salt conditions and pH, a single compromise buffer can be used that strikes a balance between conditions preferred by the various restriction enzymes. (Spec. compromise restriction buffer)
What is the function of EtBr?
Ethidium Bromide is used for visualising DNA. When EtBr binds DNA it will glow pink under UV light. This allows you to take a picture of DNA bands in a gel. The gel is soaked in an EtBr solution and then lit up by UV light. Alternatly the EtBr can be incorporated into the gel beforehand but gives a poorer picture.
What is the role of EtBr in electrophoresis?
Ethidium bromide interchalates with DNA. It doesn't affect electrophoresis, but it help visualise the DNA bands after electrophoresis. The EtBr that is bound to the DNA will fluoresce under ultraviolet light.
Why gel red can stain DNA fragments?
The DNA is a red color band under UV light on EtBr staining because EtBr intercalate and binds double stranded DNA unspecifically, when it absorbs UV light, it emits red color.
How do you get agarose gel to solidify when it doesn't solidify after 10 min in the refrigerator?
I usually add EtBr
Why is destaining done after staining in agarose gel serum electrophoresis?
To remove extra Etbr and higher background fluorescence.
Minimum DNA on agarose to visualize?
you need at least 20ng to visualize it on an agarose gel along with EtBr or GR safe
What is the function of ethidium bromide in DNA extraction?
EtBr interculates into DNA and when exposed to UV light, it causeses DNA to nick and therefore uncoil
What are DNA gels?
DNA gels is a term that usually refers to agarose gels, made with TAE (Tris, Acetate, EDTA) or TBE (Tris, Borate, EDTA) buffer. They are the simplest to make and don't contain toxic compounds (unless EtBr is added to the gel).
What is the function of TEB buffer in gel electrophoresis?
Restriction buffer maintains the pH in a range suitable for enzyme activity, as well as supplying salt cofactors required for catalysis. Since different restriction enzymes require varying salt conditions and pH, a single compromise buffer can be used that strikes a balance between conditions preferred by the various restriction enzymes. (Spec. compromise restriction buffer)
Why ethidium bromide gives orange color in UV light?
Due to fluorescence, it absorbs UV and emits Orange light.. It is due to a phenyl group.. EtBr fluoresces even when not bound to DNA but its fluorescence increases 20 times when in bound state as hydrophobic environment between base pairs force dissociation of water bound to ethidium cation. Note: Water quenches fluorescence highly.
Why do you resolve your PCR products by electrophoresis gel?
PCR products produce million copies of your gene of interest. After PCR, we usually resolve them on the agarose gel to visualize the amplified DNA using EtBr stain under UV. The main purpose is, it make sure your gene is really amplified and the length it run is corresponding to the right size of your gene of interest and purify it from other template DNA and other unspecifically amplified DNA products by extracting from the gel.
What is the principle of gel documentation system?
Purpose of Use of a gel doc system are - Photography of stained gels - Printout of photographic data - Saving of photographic data With fluorescent staining of nucleic acids, a fluorescent substance that has bound to nucleic acids is excited by ultraviolet irradiation and emits fluorescent light. The fluorescent substance EtBr binds specifically to nucleic acid and the amount of bonding depends on the molecular weight and concentration of the nucleic acid. In other words, a band for a large molecular weight or large amount will shine brighter; conversely, fluorescence will be weaker for a band for a small molecular weight or small amount.
