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Q: How does ninhydrin solution help in the visualisation of developed spots?
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Why you will spray ninhydrin for analysis in chromatography?

Since amino acids are colourless compounds, ninhydrin is used for detecting them. To identify this, after development, the TLC plate is sprayed with ninhydrin reagent and dried in an oven, at 105°C for about 5 minutes. Ninhydrin reacts with α- amino acids that results in purple coloured spots [(due to the formation of the complex - Rheuman's purple).


What is locating agent used for?

Locating agent is a substance/liquid that help to separate and analyze colorless substances in chromatography by reacting with the colorless substance to form colored spots on the chromatogram.


What happens when paper chromatography is left in the solution?

all of the spots migrate to the top, and you will not be able to tell them apart anymore.


Why you are adding marble chips during heating?

Marble chips are added during heating so that the solution or substance can boil evenly. Evenly boiling the solution will cause it not to have heating spots, which can cause an eruption of bubbles. Heating spots are caused when the heat being applied is only getting to the liquid at the bottom of the flask, causing the liquid down there to evaporate more quickly than the upper liquid. The liquid evaporating causes bubbles to shoot through the solution vigorously, disrupting the system. To avoid these heating spots, we add marble chips or boiling chips to induce a nice, even boil.


What are the dark spots called on the earth?

what are the spots on earth main or do the earth have spots what are the spots on earth main or do the earth have spots

Related questions

Why you will spray ninhydrin for analysis in chromatography?

Since amino acids are colourless compounds, ninhydrin is used for detecting them. To identify this, after development, the TLC plate is sprayed with ninhydrin reagent and dried in an oven, at 105°C for about 5 minutes. Ninhydrin reacts with α- amino acids that results in purple coloured spots [(due to the formation of the complex - Rheuman's purple).


What is the name of the small brownish spots on skin developed by exposure to sunlight?

Tan.


What are the little white spots that can developed near your eye?

They're called milia.


What is locating agent used for?

Locating agent is a substance/liquid that help to separate and analyze colorless substances in chromatography by reacting with the colorless substance to form colored spots on the chromatogram.


When iodine solution is dropped onto plant cells why do dark blue spots appear?

Dark blue spots appear to show that there is starch on the plant cells. -Anonymous-


What is the best face cream for dark spots?

Being a Dermatologist, I would suggest you to go for the best. Dark spots can be due to acne, sun or it can be your age spots. the all in solution is Lifecell. It gives you flawless and glowing skin within a week.


Why do American bulldogs develop black spots on their skin?

I have always heard that American Bulldogs inherited their black spots because of the breeding from Dalmations and certain hunting retrievers such as the English Pointer. Mostly the white dogs are the ones with the spots and as they get older the hair actually grows darker i nthose spots. My AMBD was white as a puppy and as he matued he developed brown freckles where the black spots were on his back. Of course he was covered with black spots but only developed the brown spots on his back. Hope this helps.


What is the best practice for cooling hot spots caused by dynamic loads?

Install a containment solution for dense racks


What is best practice for cooling hot spots caused by dynamic loads?

Install a containment solution for dense racks


What happens when paper chromatography is left in the solution?

all of the spots migrate to the top, and you will not be able to tell them apart anymore.


What are the red spots in sour cream?

Mold.


How do you clean water spots on prescription glasses?

To clean water spots on prescription glasses, first rinse them in cool water. Next use a commercially available lens cleaning solution. This can be bought from an eyeglass store.