Want this question answered?
You'd have to be more specific in your question. However, disks are often laden with antibiotics to see if a bacteria is resistant or susceptible to that antibiotic. The disk is placed on a nutrient agar and then bacteria is spread onto that disk. If the bacteria is susceptible to that antiobiotic, it will grow on the nutrient plate, but not around the disk containing the antibiotic. The larger the clear area around the disk, the more susceptible the bacteria is to that antibiotic. If it is resistant to that antiobiotic, it will grow on the plate and adjacent to the antibiotic laden disk indicating that antibiotic is not effective towards that particular bacterial strain. In one such test the A disk had bactracin. However, I am not comfortable saying that all A disks have bactracin. How the disk is labeled may differ.
Because nutrient agar pH is low and it has less property to antibiotic diffusion that;why we use MHA for antibiotic suseptibility testing on ph variation antibiotic does not properly work
It is the area on an agar plate where growth of a control organism is prevented by an antibiotic usually placed on the agar surface. If the test organism is susceptable to the antibiotic, it will not grow where the antibioitic is.
purity of culture, depth of seeded layer, incubation temp, agar temp, size of inoculum, distribution of inoculum, incubation period, diffusion rate of antibiotic, concentration of antibiotic on disk, growth rate of bacterium.
purity of culture, depth of seeded layer, incubation temp, agar temp, size of inoculum, distribution of inoculum, incubation period, diffusion rate of antibiotic, concentration of antibiotic on disk, growth rate of bacterium.
Zone of inhibition :-It is the area on an agar plate where growth of a control organism is prevented by an antibiotic usually placed on the agar surface. If the test organism is susceptable to the antibiotic, it will not grow where the antibioitic is. Zone of exhibition:-
why is meuller hinton(MH) agar specially used for antibiotic succeptibility testing?
the area (zone) in which bacteria cannot grow due to the presence of an antibiotic paper disk
en.wikipedia.org/wiki/Kirby-Bauer_​antibiotic_testing
Michigan
30 ÷ 17 = 1 with remainder 13.
Kirby-Bauer is the diffusion method you would use to test antibiotic sensitivity. The Mueller-Hinton is the agar plate in which you would do the method on. This is where the antibiotic discs would be places to test the sensitivity.