0
the area (zone) in which bacteria cannot grow due to the presence of an antibiotic paper disk
Wiki User
∙ 12y ago
Add your answer:
Earn +20 pts
What is the differences between zone of inhibition and zone of exhibition?
Zone of inhibition :-It is the area on an agar plate where growth of a control organism is prevented by an antibiotic usually placed on the agar surface. If the test organism is susceptable to the antibiotic, it will not grow where the antibioitic is. Zone of exhibition:-
What if a agar plate is isolated and antibiotic added and result is no growth or inhibition?
This is basically ANTIBIOTIC SENSITIVITY TEST, to test whether the given organism is RESISTANT(no zone of inhibition) or SENSITIVE( zone of inhibition) to the given antibiotic.Zone of Inhibition Testing is a fast, qualitative means to measure the ability of an antimicrobial agent to inhibit the growth of microorganisms.The effectiveness is based upon the size of zone of inhibition,diffusability of antibiotic,size of inoculum,type of media used.example: bacillus organism is inoculated with both PENICILLIN and AMPICILLIN ,zone of inhibition is absent in case of penicillin and present in case of ampicillin, this shows that ampicillin (sensitive) worked effectively when compared to penicillin.
If a petri dish shows a large zone of inhibition what does this say about the antibiotic?
That it is effective, for one thing.
Why a few bacterial colonies growing within the zone of inhibition?
bc the adapt to the new environment and become resistant to antibiotic
Why have zone inhibition?
It is the area on an agar plate where growth of a control organism is prevented by an antibiotic usually placed on the agar surface. If the test organism is susceptable to the antibiotic, it will not grow where the antibioitic is.
Please define resistant colony from a microbiology standpoint?
Resistant colonies are small colonies found in the zone of inhibition around an antibiotic disk.
What factors affect the zone of inhibition?
Pathogen susceptibility influences the zone of inhibition because organisms will not grow if they are susceptible to antibiotics. Another influence are the pH levels of agar which should fall between 7.2 and 7.4 room temperature.
How can you determine whether the zone of inhibition is due to death of a bacterium or to inhibition of growth?
How can you determine whether the zone of inhibition is due to death or the effect of the antibiotics?
Why is a larger zone of inhibition better than a small zone of inhibition?
A larger zone of inhibition means that the applied agent has either inhibited or killed the organisms that were spread on the plate and that those organisms are susceptible to that agent. In other words, a larger zone of inhibition means that the applied agent is more effective in killing/inhibiting the bacteria around it.
What are three ways and antibiotic destroys bacteria?
There are actually several common antibiotic targets. However, the three most common are the inhibition of cell wall synthesis (penicillins, cephalosporins), inhibition of protein synthesis (macrolides, tetracycline), and the inhibition of replication and transcription, (fluoroquinolones rifampin).
How do you find antibiotic assay?
For determining the type of causal agent of infectious diseases and for checking their sensitivity to antibiotics and chemotherapeutic agents in vitro by means of the inhibition zone determination method. The antibiogram allows rational and selective chemotherapy. The test discs can be coated with chemotherapeutic agents, placed on the innoculated nutrient agar and incubated. The size of the inhibition zone is a measure for the effectiveness of the substances.
How to determine whether zone of inhibition is due to death of the bacteria or its inhibition?
If the compound you are testing is bacteriocidal, you will not be able to recover bacteria from the zone of inhibition. If the compound is only bacteriostatic, you should be able to recover bacteria from the zone of inhibition by scraping the surface of the agar and resupending the scrapings in sterile saline and then spreading an aliquot of the suspension on nutrient agar that does not contain the bacteriosttic agent.
