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Hi,
I assume you mean gel electrophoresis of proteins (commonly done in a polyacrylamide gel e.g. SDS-PAGE) or agarose gel electrophoresis of DNA.
Generally, as electrophoresis is allowed to proceed for a long time, the gel and the buffer in which it is submerged in becomes heated (due to Joule heating effects of the current supply). The heating causes the pores in the gel matrix to lose their definition (due to flaccidness induced upon the polyacrylamide / agarose matrix strands within the gel) and the sample molecules (being electrophoresed) can now easily 'force' their way through the meshwork of fibres within the gel, thus creating an illusionary aspect of 'enhanced rate of migration' (i.e. 'increased rate of electrophoresis').
Hope this answers your query.
Thanks and Regards,
What else can I help you with?
What is the electrophoresis?
A method of separating substances, especially proteins, and analyzing molecular structure based on the rate of movement of each component in a colloidal suspension while under the influence of an electric field.
Some viva voce questions for electrophoresis?
1. WHAT IS ELECTROPHORESIS AND WHAT ARE THE IMPORTANTAPPLICATIONS OF ELECTROPHORESIS?Ans. Movement of charged particle in the electric field either towards cathode or anode whensubjected to an electric current is called electrophoresis.The following factors influence the movement of particles during the electrophoresis.(a) Electric current.(b) Net charge of the particle.(c) Size and shape of the particle.(d) Type of supporting media.(e) Buffer solution.Important Applications of ElectrophoresisThe technique of electrophoresis is used to separate and identify the(i) Serum proteins(ii) Serum lipoproteins(iii) Blood hemoglobins2. WHAT ARE THE DIFFERENT TYPES OF ELECTROPHORESIS?Ans. (a) Moving boundary electrophoresis: This technique was first introduced by TISELIUS in 1937(b) Zone electrophoresis: In this type of electrophoresis different types of supporting mediaare used. These are;(a) Paper electrophoresis(i) Whatman filter paper(ii) Cellulose acetate(b) Gel electrophoresis(i) Agarose.(ii) Polyacrylamide gel (used for the separation of isoenzymes).(iii) SDS-PAGE.(iv) Iso-electric focussing (proteins seperated in a medium possessing a stable pH gradient).(v) Immuno electrophoresis (for the separation of immunoglobulins).
Why is a buffer solution need for electrophoresis?
Electrophoresis refers to the movement of charged particles in a fluid or gel under the influence of an electric field. It is carried out in buffer in order to complete the electron circuit flow.
What has the author A J Houtsmuller written?
A. J. Houtsmuller has written: 'Agarose-gel-electrophoresis of lipoproteins' -- subject(s): Blood protein electrophoresis, Electrophoresis, Gel electrophoresis, Lipoproteins
How exchange rate movements influence business decision?
how exchange-rate movements influence business decisions
When was Electrophoresis - journal - created?
Electrophoresis - journal - was created in 1980.
What factors influence the rate of fermentation?
Time cultures the bacteria within the yeast, thus making the bread rise.
What is a common name for gel electrophoresis?
Agarose gel electrophoresis.
What has the author B J Haywood written?
B. J. Haywood has written: 'Electrophoresis - technical applications' -- subject(s): Abstracts, Bibliography, Electrophoresis 'Electrophoresis-technical application' -- subject(s): Bibliography, Electrophoresis
What happened to the unemployment rate from 1990 to 1998?
The unemployment rate declined from 1990 to 1998. Factors like economic growth, job creation, and government policies can influence changes in the unemployment rate over time.
Why Electrophoresis power supply results in longer gel run time?
There are several factors that control the rate at which a sample moves or migrates in a gel. One of those factors is electric power supply. The larger the voltage applied, the faster the migration. However, there is an upper limit to how much voltage can be applied. If the voltage is too high, it will cause heating in the electrophoresis module and this is turn will negatively affect the integrity of the gel.
What was used before gel electrophoresis?
Before gel electrophoresis, techniques like paper electrophoresis and agarose slab gel electrophoresis were used for separating and analyzing DNA or proteins. These methods were less efficient and had lower resolution compared to gel electrophoresis.
