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It is true that the cells of an organism (lets say Human) contain the same genome derived from it's parents. During the development of the embryos, the cells started forming a cluster of specialized tissue that end up in forming organs.
Now, all theses specialized cell types or tissues differ in respect of their GENE EXPRESSION PATTERN.
House keeping genes such as GAPDH, tubulin, actin and many more that have "basic" role in cell structure and function are constitutively expressed in different cell types.
But the cell or tissue type specific genes. As an example insulin is produced only in beta cells of the islets of Langerhans in pancreas. Antibodies are made in B cells (lymphocytes), and so hormones from its glands. These are only a few example, in fact there are 30,000 genes are coded to make proteins in the human genome, but not uniformly to express across different tissues.
One of the key factor regulates these specific expression (when it is required) is the transcription factors, external environment around the cells also influence the expression.

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Q: How is it possible that the cells within a single organism all have the same DNA genome but look different and have different functions?
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