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What does a small Rf number tell you about the characteristics of the moving molecules along the chromatography paper?
The retention factor is a ratio used in columnar chromatography. A small RF number signifies that the molecule crossed a small distance from the origin of applied pigments.
What is the significance of Rf value in chromatography?
See related link below for a better explanation than mine.. I am not to familiar with Rf values, but I have an idea. Rf values are the ratio between the distance traveled by the spot of the analyte to the distance traveled by the solvent used. This is used in paper chromatography and thin layer chromatography. Rf value will allow you to compare to your unknown substance that you are trying to analyze. I will put a link the both paper and TL chromatography.
What does Rf Values in chromatography represent?
It is the rate of flow for each color. It is calculated by taking the value for the measured from the initial color mark to the solvent front (value A) and then the distance from the initial spot to each of the centers of the color marks separated (value B). You then divide B by A. For example, solvent front for black felt tip pen is 5.4cm and the distance for value B is .9 cm. The rf for this problem is .9cm/5.4 cm = .17 (Rf factor is a unitless number)
What are the variables in a plan and design paper chromatography lab?
responding variable-distance of components( Rf values)
What is Rf value?
It is the distance travelled by the sample or analyte divided by distance travelled by the solvent front in chromatography.
Does Rf means resolution factor in paper chromatography?
Rf is nothing but retardation factor in paper chromatography.Rf= distance spot traveled/distance solvent traveled
What does Rf stand for How can it be calculated?
retadartion factor calculate it by Rf= distence moved by chemical distence moved by solvent ; ;-)
What does a small Rf number tell you about the characteristics of the moving molecules along the chromatography paper?
The retention factor is a ratio used in columnar chromatography. A small RF number signifies that the molecule crossed a small distance from the origin of applied pigments.
What is the significance of Rf value in chromatography?
See related link below for a better explanation than mine.. I am not to familiar with Rf values, but I have an idea. Rf values are the ratio between the distance traveled by the spot of the analyte to the distance traveled by the solvent used. This is used in paper chromatography and thin layer chromatography. Rf value will allow you to compare to your unknown substance that you are trying to analyze. I will put a link the both paper and TL chromatography.
How do you calculate Rf in chromatography?
In chromatography, Rf is the distance the solute travels divided by the distance the mobile phase travels. For example, in thin layer chromatography, if the spot travels 7 cm, and the mobile phase travels 15 cm, the Rf value for that spot will be 7/15 = 0.47
How do you measure Rf?
Rf value = distance traveled by a compound/distance traveled by a constant.Source: http://www.chemguide.co.uk/analysis/chromatography/paper.html
What is an rf value used for?
The rf value is shorthand for the retention value of a substance. It is used in chromatography to determine the components of an unknown sample.
What are the significance of Retention factor values?
Rf Values determine the solubility of a substance with respect to a certain solvent. It also determines the affinity of the solute to the solvent (greater Rf=greater affinity of solute to the solvent)
What does Rf Values in chromatography represent?
It is the rate of flow for each color. It is calculated by taking the value for the measured from the initial color mark to the solvent front (value A) and then the distance from the initial spot to each of the centers of the color marks separated (value B). You then divide B by A. For example, solvent front for black felt tip pen is 5.4cm and the distance for value B is .9 cm. The rf for this problem is .9cm/5.4 cm = .17 (Rf factor is a unitless number)
How do you work out RF value?
Rf = distance to color/distance to front line
What is Rf value?
It is the distance travelled by the sample or analyte divided by distance travelled by the solvent front in chromatography.
What are the variables in a plan and design paper chromatography lab?
responding variable-distance of components( Rf values)
