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Sample overload will result in the formation of a streak rather than separate bands.. And confuses with the results!!!! And moreover it will get you a good name from your boss" can't even run a proper gel!!!(lol)"...
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∙ 14y ago
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What is pulsed field gel electrophoresis?
This method is a mode of gel electrophoresis in which the applied field is switched between poles so the DNA sample is constantly re oriented within the frame work of the gel. This re alignment allows the sample to move smoothly through the gel
If all the bands on an electrophoresis gel are the same color the single stranded DNA sample consisted of one kind of?
false
What holds the DNA sample during electrophoresis?
DNA samples are within the gel matrix during electrophoresis. DNA moves at differtent rates through the pores of the gel depending on how long the fragments are. DNA is held by the gel itself.
What is the purpose of a stain in electrophoresis?
I think the Question should be " What is the use of a dye in electrophoresis?" to be more specific, The normal dyes that are incorporated into the sample loading buffer in case of agarose gel is bromophenol blue and xylene cyanol.These are used to track the movement of the sample, so that we can stop the electrophoresis when the dye front is nearly 80%(can vary depending on the molecular weight of the sample) away from the well. In case of polyacrylamide gel electrophoresis the dye has many functions: gives density to the sample so that it sits properly in the well. SDS binds to the amino acid and makes it anionic, so that the movement of the sample is based on molecular weight, if not the charge of the protein will also influence the movement. it also helps us to track the movement of the sample. DTT or mercaptoethanol helps in the breaking of cysteine bonds.. That's it.. But the question is too broad and can be specific!!!!!!!!!1
What effect does the amount of the sample of a substance affect its density?
it has no effect. density of a substance is the same no matter the size or shape of the sample.
How does gel electrophoresis is used to make a DNA fingerprint?
Gel electrophoresis separates DNA fragment on the basis of their size. In DNA fingerprinting or DNA typing given sample is cut up with restriction enzymes and run through electrophoresis and results are analyzed to check for DNA polymorphism between the given sample and a sample form suspect. In nutshell gel electrophoresis is boon for the people in forensics.
What is hemoglobin electrophoresis?
This test requires a blood sample. No special preparation is needed before the test.
What is pulsed field gel electrophoresis?
This method is a mode of gel electrophoresis in which the applied field is switched between poles so the DNA sample is constantly re oriented within the frame work of the gel. This re alignment allows the sample to move smoothly through the gel
WHAT CAN be done to change the running speed of sample and resolving power of gel on electrophoresis?
Fresh buffers are responsible for speed and powerful migration, also the voltage in which the sample is running matters!
If all the bands on an electrophoresis gel are the same color the single stranded DNA sample consisted of one kind of?
false
What holds the DNA sample during electrophoresis?
DNA samples are within the gel matrix during electrophoresis. DNA moves at differtent rates through the pores of the gel depending on how long the fragments are. DNA is held by the gel itself.
What is the purpose of a stain in electrophoresis?
I think the Question should be " What is the use of a dye in electrophoresis?" to be more specific, The normal dyes that are incorporated into the sample loading buffer in case of agarose gel is bromophenol blue and xylene cyanol.These are used to track the movement of the sample, so that we can stop the electrophoresis when the dye front is nearly 80%(can vary depending on the molecular weight of the sample) away from the well. In case of polyacrylamide gel electrophoresis the dye has many functions: gives density to the sample so that it sits properly in the well. SDS binds to the amino acid and makes it anionic, so that the movement of the sample is based on molecular weight, if not the charge of the protein will also influence the movement. it also helps us to track the movement of the sample. DTT or mercaptoethanol helps in the breaking of cysteine bonds.. That's it.. But the question is too broad and can be specific!!!!!!!!!1
Which of the following best describes how scientists use gel electrophoresis?
Gel Electrophoresis is the technique for sorting DNA molecules based on their number of base pairs by running a current through the gel. The sample travels opposite of the direction of the electrical charge since DNA is negatively charged. Using a certain primer you can identify the BP of the sample.
What is used to sort DNA into different lenghts?
Gel Electrophoresis
If you were called away during the electrophoresis procedure and were not able to monitor your sample run what would happen if the electricity were to remain running in your absence?
you would die
What effect does the amount of the sample of a substance affect its density?
it has no effect. density of a substance is the same no matter the size or shape of the sample.
Why stacking gel used in electrophoresis?
Stacking gel is used to make a thin uniform band of the DNa sample before the real seperation takes place.
