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Heat fixed; because it kills the bacteria,firmly affix the smear to the microscope slide, and allow the sample to more readily take up the stain.
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∙ 11y ago
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Why do you air-dry a smear?
To avoid denaturing and destroying the smear.
Why is it often necessary to use a cover slip when using the microscope?
Coverslip is not placed on a microscope but on the stained specimen on slide . This protects objective lens of microscope from getting stain from a wet mount . It also protects permanent slide .
When observing a slide should the specimen be on the top or bottom surface of the microscope?
A specimen should be on the top surface of the slide and place a cover-slip over it.
Why is there need to fix the bacteria to the slide?
To heat fix a bacterial smear you would put the specimen on the slide and either put slide on a slide warmer or over a Bunsen burner for a few seconds. Heat fixing a bacterial smear does kill the specimen but it makes the bacteria stick the slide to withstand the rinsing process.
Is presence of grease or dirt on a glass slide going to result in poor smear preparation?
The smear will not adhere well if there is grease on the slide.
What is the use of cover slip or cover glass?
to protect the smear or specimen from damage.
Why use dye in preparation of cheek smear specimen?
it makes the organelles of the cells stand out
What is the function of methanol in the wright's stain solution?
to fixed the blood smear
What is a consequence of not leaving a stain on the smear long enough?
The specimen will?ænot absorb adequate stain, resulting in little contrast. Thus making it difficult for identification of?ædifferent components of the specimen.
What should you do if a specimen spills on your clothes?
What is the specimen?
Is a pap smear 100 percent accurate?
No it is not. There are various places for errors. The specimen may not be perfect, and there are possible errors in the processing too.
Why do you air-dry a smear?
To avoid denaturing and destroying the smear.
What would you expect from a gram stain done on a slide that was heated too hot during the heat-fixed smear?
A Gram stain refers to a staining technique for the identification of bacteria. A Gram stain done on a slide that was heated too hot during the heat-fixed smear will destroy the cell wall of the bacteria.
If you are a virgin should a pap smear still be recommended?
No
Why is it often necessary to use a cover slip when using the microscope?
Coverslip is not placed on a microscope but on the stained specimen on slide . This protects objective lens of microscope from getting stain from a wet mount . It also protects permanent slide .
Should you always use sterilized water for smear preparation?
Yes, when you are performing smear preparation, you should always use sterilized water. This will ensure that nothing contaminates your slide.
When observing a slide should the specimen be on the top or bottom surface of the microscope?
A specimen should be on the top surface of the slide and place a cover-slip over it.
