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it makes the organelles of the cells stand out
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What dye is used to stain cheek epithelial cells in lab?
methane blue
What is the function of dye in the preparing of specimen under microscope?
The dye shows up certain cell structures that are different from say the main cells your wanting to look at . The type of dye used is what counts
Why is it you should heat-fixing the air-dried before staining?
It is used to fix because to make the cell inactive or immoblie, but the main purpose is to fix the smear so that when we put stain and then flush it out with water ( or some time with alcohol) the smear should not wash out with dye.
What is the effect of pH in the preparation of dye?
The effect of it is so that the person doing the experiment knows exactly if the chemical is acidic or alkeline.
What is gram positive and gram negative?
Gram-positive is a result of the Gram staining technique, developed by Danish scientist Hans Christian Gram (1853 - 1938). Bacteria can have two types of cell walls. Gram-Positive bacteria have a relatively thick layer of peptidoglycan. Gram-negative bacteria have a much thinner layer of peptidoglycan. There are 4 basic steps to a Gram-staining. # The smear is flooded with a primary stain (such as crystal violet). This generally ends up staining all cells within the smear. # The smear is rinsed to remove excess dye, and a mordant such as an iodine solution is flooded onto the smear. A mordant is a substance that increases the affinity of cellular components for a dye. # The smear is rinsed again, removing all excess dye. The smear is then briefly washed with a 95% alcohol or a alcohol-acetone mixture. This mixture acts as a decolorizing agent. If the color is washed away then you are dealing with a Gram-negative bacteria (thin layer of peptidoglycan). # A counterstain is applied to the rest of the smear as a contrasting color to the now colorless Gram-negative bacteria (typically the red dye safranin). The Gram-positive bacteria remains violet because the dye was never decolorized because of the thick peptidoglycan cell wall. The gist of this is that Gram-positive bacteria will absorb the dye within their thick peptidoglycan cell wall component and resist the effects of decolorizing alcohol. Gram-negative bacteria will easily lose the dye from their thin peptidoglycan component of the cell wall. The significance of this test allows Microbiologists, Doctors, etc to fight the bacteria with certain specific antibiotics.
What is it called to add dye to a specimen under a microscope?
to stain.
What dye is used to stain cheek epithelial cells in lab?
methane blue
What is active ingredient in the dye preparation that will lighten your hair?
Hydrogen peroxide
What is the function of dye in the preparing of specimen under microscope?
The dye shows up certain cell structures that are different from say the main cells your wanting to look at . The type of dye used is what counts
Why doesn't a negative stain colorize the cells in the smear?
A negative stain will stain the background with an acidic dye, such as Nigrosin. This procedure is used to demonstrate capsules. This technique brings the specimen off of the background for more adequate viewing purposes.
What is the preparation of smears and simple staining?
smear is the putting and fixing of staining sample on glass slide which is done by first putting the a drop of water on slide and then inoculation is put over it which is then spread slowly in round form by inoculating loop and dry it by very light heat to fix it.Simple staining is the process in which a dye knwon as methylene blue is spread over smear to colour the microbe whcih can be then washed by 70% alcohol so that extra dye can be removed and then the sample is ready to observe under microscope
Why is methylene blue necessary to view your check cells?
This is because the cheek cell is transparent. Since the membrane of the cheek cell is selectively permeable, it allows the methylene blue to enter the cell , therefore makes it blue in color from the inside. Then we are able to see the cheek cell properly under a microscope... please note. : we are supposed to add a bit of water and gliserene too . WATER : to transfer the cheek cells on to the slide AND GLISERENE: to prevent the cheek cells from drying up.
Why is it you should heat-fixing the air-dried before staining?
It is used to fix because to make the cell inactive or immoblie, but the main purpose is to fix the smear so that when we put stain and then flush it out with water ( or some time with alcohol) the smear should not wash out with dye.
Why is it necessary to excessively rinse the smear with water in experiment identifying bacteria using gram stain?
smear should be rinsed with distill water so that all the small particles attach to the smear will washed away such as some time the crystals of dye are attached to the smear which give the illusion of microbial cell some time, distill water is used because it is free from other microbial cell and ions which can harm the smear.
What is the effect of pH in the preparation of dye?
The effect of it is so that the person doing the experiment knows exactly if the chemical is acidic or alkeline.
Can cells be stained with dye?
Live staining is possible as is the preparation of fixed tissue.
What is gram positive and gram negative?
Gram-positive is a result of the Gram staining technique, developed by Danish scientist Hans Christian Gram (1853 - 1938). Bacteria can have two types of cell walls. Gram-Positive bacteria have a relatively thick layer of peptidoglycan. Gram-negative bacteria have a much thinner layer of peptidoglycan. There are 4 basic steps to a Gram-staining. # The smear is flooded with a primary stain (such as crystal violet). This generally ends up staining all cells within the smear. # The smear is rinsed to remove excess dye, and a mordant such as an iodine solution is flooded onto the smear. A mordant is a substance that increases the affinity of cellular components for a dye. # The smear is rinsed again, removing all excess dye. The smear is then briefly washed with a 95% alcohol or a alcohol-acetone mixture. This mixture acts as a decolorizing agent. If the color is washed away then you are dealing with a Gram-negative bacteria (thin layer of peptidoglycan). # A counterstain is applied to the rest of the smear as a contrasting color to the now colorless Gram-negative bacteria (typically the red dye safranin). The Gram-positive bacteria remains violet because the dye was never decolorized because of the thick peptidoglycan cell wall. The gist of this is that Gram-positive bacteria will absorb the dye within their thick peptidoglycan cell wall component and resist the effects of decolorizing alcohol. Gram-negative bacteria will easily lose the dye from their thin peptidoglycan component of the cell wall. The significance of this test allows Microbiologists, Doctors, etc to fight the bacteria with certain specific antibiotics.
