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What is the purpose of staining the smear in malachite green during spore staining?
the purpose of boiling of smear in malachite green is to forces a stain to penetrate the endospore wall, it is necessary to heat the slide and the stain to prod the wall to allow the stain to enter.
Is there any difference if the prepared smear is too thick or too thin?
Thin smears of blood are needed to investigate hematological problems or disorders of the blood. It is also used to identify the parasite within the blood. Thick films enables the microscopist to screen the blood of a larger volume. They are more sensitive than the thin film.
What is a cervical smear?
A cervical smear, also known as a Pap smear, is a screening test used to detect abnormalities in the cells of the cervix that could potentially lead to cervical cancer. During the procedure, cells are collected from the cervix and examined under a microscope for any signs of pre-cancerous or cancerous changes. It is a routine test recommended for women to help prevent cervical cancer.
Why is it important to limit the quantity of cells used to prepare smear?
By applying to many cell in a smear it would become difficult to study the morphology of single cell as they will form clumps for e.g. if we want to study some cocci then it would be difficult to estimate there occurence i.e. either they are in chain or in cluster or may be diplococci or monococci.
Reasons why a smear from a gram-positive control showed a gram-negative result?
Possible reasons for a gram-positive control smear showing a gram-negative result could include errors in the staining process, contamination of the specimen with gram-negative bacteria, or misidentification of the control as a gram-positive organism when it is actually gram-negative. Additional troubleshooting and repeat testing may be needed to confirm the result.
What are reactive glandular cells in a pap smear?
epithelial cells
What is the purpose of staining the smear in malachite green during spore staining?
the purpose of boiling of smear in malachite green is to forces a stain to penetrate the endospore wall, it is necessary to heat the slide and the stain to prod the wall to allow the stain to enter.
What are polymorphonuclear cells as result in a pap smear test?
Polymorphonuclear cells are white blood cells. These are a nonspecific finding on a pap smear result. Contact your health care provider to find out if any further evaluation is required in your specific situation.
What does it mean when you have epithelial cells present in a pap smear?
Epithelial cells are a normal part of the anatomy. They are the type of cell checked in a pap smear. If they are present, it's a sign that the pap smear got a decent sample.
Why dense smear not a good smear preparation?
A dense smear will make it difficult to evaluate cells, as they may be all "piled up" and hard to evaluate.
How do you classify a pap smear?
A pap smear is classified in a number of ways. The adequacy of the smear is evaluated in terms of the types and numbers of cells collected. The cervical cells are evaluated for normalcy. Lastly, if any additional findings are noted, such as yeast, these are reported as well.
What does it mean if pap smear results show endocervical and or squamous metaplastic cells are present?
Endocervical and/or squamous cells are normal on a pap smear. This notation on your result means the test was complete.
What is htb mean in a pap smear?
what is HTB with Pap,and celula,negative.
What kind of cells does a pap smear detect?
ASC-US. ASC-H ,AGC ,AIS,LSIL ,AND HSIL cells Detect
Staining preparation with eosin and methylene blue?
Eosin is a red stand and methylene blue is blue. The result of staining a bacterial smear with a mixture of eosin and methylene blue is that eosin is acidic and acts as a negative stain. Methylene blue is basic the smear background would turn out red while the cells would turn out blue.
Is there any difference if the prepared smear is too thick or too thin?
Thin smears of blood are needed to investigate hematological problems or disorders of the blood. It is also used to identify the parasite within the blood. Thick films enables the microscopist to screen the blood of a larger volume. They are more sensitive than the thin film.
What happens to Smudge cells in an albumin smear?
Cell fragility causes smudge cells to appear on peripheral smears. Addition of albumin prior to slide preparation essentially coats the fragile cells, thus keeping them intact on smear preparation.
