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A negative stain will stain the background with an acidic dye, such as Nigrosin. This procedure is used to demonstrate capsules. This technique brings the specimen off of the background for more adequate viewing purposes.
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In gram staining why will very thick smears give inaccurate results?
If a smear exhibits uneven thickness, overlapping cells may not get the proper exposure to the reagents. This results in uneven or mottled staining. For example, in the thicker areas of the smear, gram-negative cells may not decolorize sufficiently and end up staining purple.
What is a cervical smear?
A cervical smear is a screening test used to detect pre-cancerous and cancerous cells by taking a sample of cells from the cervix.
Reasons why a smear from a gram-positive control showed a gram-negative result?
the smear decolourised more time than it required
What is blood smear?
A blood film or peripheral blood smear is a microscope slide made from a drop of blood, that allows the cells to be examined microscopically
Why is it important to limit the quantity of cells used to prepare smear?
By applying to many cell in a smear it would become difficult to study the morphology of single cell as they will form clumps for e.g. if we want to study some cocci then it would be difficult to estimate there occurence i.e. either they are in chain or in cluster or may be diplococci or monococci.
What are reactive glandular cells in a pap smear?
epithelial cells
What are polymorphonuclear cells as result in a pap smear test?
Polymorphonuclear cells are white blood cells. These are a nonspecific finding on a pap smear result. Contact your health care provider to find out if any further evaluation is required in your specific situation.
What does it mean when you have epithelial cells present in a pap smear?
Epithelial cells are a normal part of the anatomy. They are the type of cell checked in a pap smear. If they are present, it's a sign that the pap smear got a decent sample.
In gram staining why will very thick smears give inaccurate results?
If a smear exhibits uneven thickness, overlapping cells may not get the proper exposure to the reagents. This results in uneven or mottled staining. For example, in the thicker areas of the smear, gram-negative cells may not decolorize sufficiently and end up staining purple.
Why dense smear not a good smear preparation?
A dense smear will make it difficult to evaluate cells, as they may be all "piled up" and hard to evaluate.
What is gram variability?
If after staining, a smear reveals both purple(Gram +) and red cells(Gram -). This is generally an indication that the smear contains gram positive cells.
What is a cervical smear?
A cervical smear is a screening test used to detect pre-cancerous and cancerous cells by taking a sample of cells from the cervix.
Reasons why a smear from a gram-positive control showed a gram-negative result?
the smear decolourised more time than it required
How do you classify a pap smear?
A pap smear is classified in a number of ways. The adequacy of the smear is evaluated in terms of the types and numbers of cells collected. The cervical cells are evaluated for normalcy. Lastly, if any additional findings are noted, such as yeast, these are reported as well.
What does it mean if pap smear results show endocervical and or squamous metaplastic cells are present?
Endocervical and/or squamous cells are normal on a pap smear. This notation on your result means the test was complete.
What is htb mean in a pap smear?
what is HTB with Pap,and celula,negative.
Why is it important to include both gram negative and gram negative organisms in a smear prep when you are performing the gram stain?
The point of the gram stain is to differentiate between two things, with out both positive and negative gram cells there would be nothing to differentiate between, defeating the purpose of the process.
