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use cryopreservation technique..

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Q: How to prepare permanent and working stock culture?
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Prepare a working solution from a stock solution?

it is very easy to prepare working solution from a stock solution we use the formula for this purpose which is: C1V1 = C2V2 C1 is the concentration of the stock solution V1 required volume from the stock solution C2 concentration of the working solution V2 volume of the working solution


How would you prepare tetracycline stock solution?

10 mg/ml stock solution


How do you prepare 5mg percent working uric acid standard solution from stock uric acid standard solution?

Preparation of standards Prepare a stock uric acid solution of 100 mg/L. Dilute it to give working concentrations of 10, 20, 30, 40, 50 and 60 mg/L. Procedure:1 Prepare a 100 mg/L stock solution of uric acid Weigh out 100 mg uric acid, transfer it to a 1 L volumetric flash, add about 900 ml distilled water, and then add about 100 µl of 0.6 N NaOH to help dissolve the uric acid. Make up to the volume with water when uric acid is fully dissolved.2 Dilute the stock solution to give working concentrations of 10, 20, 30, 40, 50, 60 mg/L. To prepare 50 ml of the working standards 5, 10, 20, 30, 40, 50 mg/L respectively, weigh 10, 15, 20, 25, 30 g of stock solution into 50 ml volumetric flasks and make up to volume with distilled water.


How do you prepare 10 mm solution with 4 m stock solution?

10-4


You are given 10mg of powdered retinoic acid. you must make up a suitable stock concentration so that you can dilute that into one of your wells so that it is at a final concentration of 3uM?

Dissolve it to 100mM in DMSO, aliquot and store at -80℃. Upon use, take out one aliquot and dilute it to 1mM in absolute ethanol, and supplement the culture medium accordingly. Store this working stock under -20℃ for up to 2 weeks.

Related questions

Prepare a working solution from a stock solution?

it is very easy to prepare working solution from a stock solution we use the formula for this purpose which is: C1V1 = C2V2 C1 is the concentration of the stock solution V1 required volume from the stock solution C2 concentration of the working solution V2 volume of the working solution


Is capital stock a permanent or temporary account?

Capital stock is considered a permanent account. Permanent accounts are ones which hold financial information for multiple accounting periods. Capital stock remains in an account until an accountant moves it to another account, which means that it is permanent.


How would you prepare tetracycline stock solution?

10 mg/ml stock solution


How do you prepare report of stock verification of library?

To prepare a report of stock verification of library, the most recently recorded worth and revenue generated by the library must be gathered and averaged out.


How do you prepare 5mg percent working uric acid standard solution from stock uric acid standard solution?

Preparation of standards Prepare a stock uric acid solution of 100 mg/L. Dilute it to give working concentrations of 10, 20, 30, 40, 50 and 60 mg/L. Procedure:1 Prepare a 100 mg/L stock solution of uric acid Weigh out 100 mg uric acid, transfer it to a 1 L volumetric flash, add about 900 ml distilled water, and then add about 100 µl of 0.6 N NaOH to help dissolve the uric acid. Make up to the volume with water when uric acid is fully dissolved.2 Dilute the stock solution to give working concentrations of 10, 20, 30, 40, 50, 60 mg/L. To prepare 50 ml of the working standards 5, 10, 20, 30, 40, 50 mg/L respectively, weigh 10, 15, 20, 25, 30 g of stock solution into 50 ml volumetric flasks and make up to volume with distilled water.


What types of jobs are available in working with stocks or in the stock market?

What types of jobs are available in working with stocks or the stock market?


What jobs are available in the stock market and what courses of study should you take to prepare for them?

There are numerous jobs to be found in the stock market. In order to enroll in courses to prepare you for one, you must first decide what it is you are interested in. Then you would be able to begin to prepare yourself for the studies necessary.


You prepare a less concentrated H C l solution from a stock solution with 12m concentration. If you too 100g of the stock solution to prepare 4 MHCl solution how much water is needed to prepare o find solution 9density HCL(12) = 1,89/ml?

Image result for You prepare a less concentrated H C l solution from a stock solution with 12m concentration. If you too 100g of the stock solution to prepare 4 MHCl solution how much water is needed to prepare o find solution 9density HCL(12) = 1,89/ml? The concentration would be 0.76 mol/L.


Where can I learn about stock option administration?

Stock Option Administration are contracts that a lawyer would prepare for you when you are investing in the stock market. You can find information about these at salary.com and other various websites.


What is Dells stock exchange?

the dell stock exchange is the fundimental process to working through


Why should you make stock culture of bacteria?

For research purposes


What is wallstreet?

Wall Street was the first permanent home of the New York Stock Exchange.