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MTT =3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide
The assay method was developed by using high performance liquid chromatography. The plasma is separated by passing through an ultra filter.
an assay that measures the appearance or disappearance of a fluorescent.
Formic acid can be determined by ion chromatography.
it is a assay method which we can find the growth of fungi.
spectro assay of serratiopeptidase
The most common assay used to measure invertase activity is the DNS method, which involves measuring the release of reducing sugars from sucrose using dinitrosalicylic acid. This assay is based on the colorimetric detection of reducing sugars, which allows for the quantification of enzyme activity. Other methods include the Nelson-Somogyi method and the Nelson-Somogyi 3,5-dinitrosalicylic acid method.
non-destructive
The tributyrin assay method is a biochemical technique used to evaluate the lipase activity of microorganisms, particularly in the context of enzyme production. In this method, tributyrin, a triglyceride, is used as a substrate, and the breakdown of tributyrin by lipase results in the release of free fatty acids, which can be quantified. The assay typically involves measuring the change in turbidity or pH, or using colorimetric methods to determine enzyme activity. This method is widely applied in research and industrial contexts to assess the efficiency of lipase-producing organisms.
The tartaric assay method falls under the alkalimetric direct titration method because it involves the direct titration of tartaric acid with a standardized alkali solution to determine its concentration. The method is based on the neutralization reaction between the tartaric acid and the alkali, which allows for the quantitative determination of the tartaric acid content present in the sample.
The procedures for carrying out spectrophotometric and fluorometric GUS assays are similar in that they both involve measuring the enzymatic activity of the GUS enzyme, but they differ in the detection method. In the spectrophotometric assay, absorbance changes at a specific wavelength are measured, while the fluorometric assay measures fluorescence emitted from a substrate after the GUS enzyme acts on it. Additionally, the sensitivity and specific conditions for each assay may vary, influencing the choice of method based on the experimental requirements.
The classical method for preparing CuBr2 involves reacting copper oxide or carbonate with hydrobromic acid. This reaction forms copper bromide and water as products. The balanced chemical equation for this reaction is CuO + 2HBr -> CuBr2 + H2O.