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What would be the result of overheating the bacterial smear?
Overheating the bacterial smear can result in distortion or destruction of the bacterial cells, making it difficult to observe them under the microscope. This can lead to inaccurate or inconclusive results when trying to identify the bacteria present on the smear.
What is the purpose of covering the smear with bibulous paper when performing the endospore stain?
Covering the smear with bibulous paper during the endospore stain process helps to wick away excess stain and prevent the slide from drying out. This ensures that the endospores are properly stained and the background is clear for observation under the microscope.
What is the Result of over heating the bacterial smear?
When you heat the bacteria more than three times on the flame of Bunsen burner, the bacteria will damage and if you stain this damaged bacteria, the shape of bacteria is not typical and sometimes you just see the residue of stain on the slide.
What is the purpose of simple staining procedure?
In simple staining the bacterial smear stained with single dye or reagent which bring the distinctive contrast between organism and its background. PROCEDURE: 1 Prepare bacterial smear by placing loopfull culture of bacteria on slide. 2 Allow the slide to dry and heat fix it over flame. Do not heat extreamly, it can demage the shape or structure of bacteria. 3 Now flood the smear with methylene blue for 1-2 minutes. 4 Gently wash the smear with distill water to remove excess stain and dry it slowly with tissue paper. 5 Examine the slide in 100x or oil-immersion for the morphology of bacteria.
Is presence of grease or dirt on a glass slide going to result in poor smear preparation?
The smear will not adhere well if there is grease on the slide.
What would be the result of overheating the bacterial smear?
Overheating the bacterial smear can result in distortion or destruction of the bacterial cells, making it difficult to observe them under the microscope. This can lead to inaccurate or inconclusive results when trying to identify the bacteria present on the smear.
What are the characteristics of good bacterial smear?
no thing
What are the disadvantages of a poorly prepared bacterial smear?
Disadvantages of a poorly prepared bacterial smear include distorted bacterial morphology, overlapping cells, uneven distribution of cells, and difficulty in interpreting microscopy results accurately. These issues can lead to misidentification of bacteria and inaccurate assessment of their characteristics.
When observing the prepared bacterial smears at the 1000X magnification should your iris diaphragm be all the way open or stopped down for minimal light?
The iris diaphragm should be partially closed to allow for optimal contrast when observing bacterial smears at 1000X magnification. This setting helps improve visibility of the specimen by reducing glare and enhancing details in the sample.
What is the purpose of covering the smear with bibulous paper when performing the endospore stain?
Covering the smear with bibulous paper during the endospore stain process helps to wick away excess stain and prevent the slide from drying out. This ensures that the endospores are properly stained and the background is clear for observation under the microscope.
Is a bacterial infection test the same as a chlamydia test?
Testing for chlamydia is very specific. A regular bacterial culture or wet smear will not detect chlamydia.
Why does the physician take a Pap smear?
Such swab tests are used to check for gonorrhea and chlamydia, or bacterial vaginitis, which is a bacterial infection resulting in inflammation of the vagina.
What happens if you don't heat fix a smear prior to staining of a bacterial slide?
If a bacterial smear is not heat fixed prior to staining, the bacteria may not adhere well to the slide and can wash away during the staining process. Heat fixing helps to kill the bacteria, firmly attach them to the slide, and improve the uptake of stain, resulting in better staining results. Without heat fixing, the bacteria may not stain properly or may not be visible at all under the microscope.
Give the reason for passing bacterial smear through the flame before staining?
Passing the bacterial smear through the flame before staining is done to heat-fix the bacteria onto the slide, making them adhere firmly and preventing them from washing off during the staining process. Heat fixing also kills the bacteria, which helps in the preservation of their cellular structures for visualization under the microscope.
What is the Result of over heating the bacterial smear?
When you heat the bacteria more than three times on the flame of Bunsen burner, the bacteria will damage and if you stain this damaged bacteria, the shape of bacteria is not typical and sometimes you just see the residue of stain on the slide.
What is cocobacilli in a pap smear?
Coccobacilli on a pap smear result is informational. If the woman is comlplaining fo vaginal discharge and odor, treatment for bacterial vaginosis is offered. If she has no complaints, treatment is not needed.
At what total magnification should you begin to look at a sample?
Depending on what microscopy you are doing.. Bacterial microscopy starts with 40x and Blood smear microscopy at 10x.
