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How you prepare 50mM of TES buffer pls reply as soon as possible?
Seemakrishna
Is that 50 millimolarity? Thin that would be 0.05 Molarity
Molarity = moles of solute/Liters of solution
so, one needs to know how much volume or how much mass to help you.
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∙ 13y ago
Prof.Dr.Nawras Abdel...
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How would you make 1l using 10mm tris hcl 50mm kcl 1.5mm mgcl2 ph8 3 25 degreesc?
Need calculation on how to make this buffer
What is the osmolarity of 50mM of glucose?
50m osmoles
Function of GTE buffer solution?
GTE stands for Glucose-Tris-EDTA.Glucose is used to maintain osmolarity: 50mM (millimolar) glucose prevents premature cell lysis, which can cause lower DNA yields to due to aggregation and degradation. The other components of the buffer also contribute to the osmolarity of the solution, but glucose, being a non-electrolyte, is a good choice because it does not interfere with the solution's buffer properties. The Tris is used to buffer whatever you're adding this to at pH 7.9. EDTA binds divalent cations like Ca2+ and Mg2+, thereby weakening the cell envelope of cells in the mixture. This is typically used for miniprep/DNA purification, when you have to lyse the cell and get internal cell components out into the solution.
How prepare 50mM naoh?
One mole of NaOH weighs 23(Na)+16(O)+1(H)=40 g A 0.05 M NaOH is then 2 g NaOH/L. So dissolve 2 g NaOH in 500 ml water let it cool down to room temperature and fill it up to one liter. For accurate results you can use an analytical balance and a volumetric flask.
How do you prepare 50mM TES buffer?
In order to prepare 50mM TES buffer, you will need to add in approximately 1000 ml of Proteinase K solution. From there, you will need to separate and stack the gels.
How would you make 1l using 10mm tris hcl 50mm kcl 1.5mm mgcl2 ph8 3 25 degreesc?
Need calculation on how to make this buffer
What is 50mm?
It is: 50mm = 5cm
Who makes the best 50mm Lens?
Canon makes the best 50mm camera lens, known as the EF 50mm f/1.8mk2, 50mm f/1.4 USM and 50mm f/1.2L lenses
What is 50mm from 0.14 mm?
50mm from 0.14mm = -49.86mm
How many centimetre in 50mm?
10mm = 1cm 50mm = 5cm
What is 168cm add 50mm?
168cm add 50mm = 218
How many 50mm are in cm?
50mm=5cm 10mm=1cm
What are 50mm drivers?
50mm drivers could be loudspeakers. (Tweeters)
Who makes the best lens?
Canon makes the best 50mm camera lens, known as the EF 50mm f/1.8mk2, 50mm f/1.4 USM and 50mm f/1.2L lenses
What is the magnification factor for a 50MM lens?
A 50mm lens has a diopter of 20.
Which is the greater length 50mm or 6cm?
Between 50mm and 6cm, the later would have the greater length. That is because 50mm is only equal to 5cm.
