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Glutamic acid production by microbial fermentation provides 90% of world's total demand, and remaining

10% is met through chemical

methods. The raw materials used include carbohydrate (glucose, molasses, sucrose, etc.), peptone, inorganic salts and biotin. Biotin concentration in the fermentation medium

has a significant influence on the yield of glutamic acid. Fermentation completes within 2-4 days and the yield is considered

as high as 50 mg/ml of glutamic acid.

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