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Is dh5 alpha srain of E. coli is dam negative?
no
What is the significance of E. coli DH5 alpha?
E. Coli DH5 alpha cells are mainly of value to scientific research. They are frequently used for transformation of plasmid DNA, which is a research tool for amplifying the amount of plasmids. One can insert a gene of interest into a plasmid but the yield of this process is limited. If you were then to insert this plasmid into a living cell, your plasmid would be copied with every division of that cell. Bacteria normally host plasmids and are not too picky about it so one more is usually not a problem. Since bacteria like E. coli are really easy to grow, maintain and store, the DH5 alpha strain is perfect. As far as technical details about the strain go: DH5 alpha have been made deficient in some genes, which protects foreign DNA for instance. endA1 gene in DH5 alfa is modified (mutated) so that the intracellular endonuclease it code is inactive that degrades plasmid DNA in many preparations
What is the difference between DH5 alpha and BL21 with reference to cloning?
BL21 is RecA+, whereas DH5alpha is RecA- and thus more appropriate for cloning purposes. BL21 also produces lower levels of proteases and thus better for expression of recombinant proteins.
You have taken two control.One with LB media only and other with LB plus kanamycin.LB plus kanamycin showed growth upon incubation. what could be the reason?
This means the bacteria that you are handling is resistant to kanamycin and can therefore grow in the presence of this antibiotic. You have to check if there are colonies on the control plate containing only LB medium. If there are colonies, then this reasoning is valid. A possible reason why bacteria can grow in the presence of kanamycin is they could have a plasmid that is conferring antibiotic resistance. However, this is only a hypothesis and further investigation (like plasmid isolation) is required to confirm it.
Alternative to use of kanamycin for surgical irrigation-thanks?
I dunno, we are in the same situation with the temporary discontinuation of Kanamycin. We will most likely switch to Amikacin.
What is kanamycin sulfate?
It is an oral anti-infective
What does kanamycin target?
the 30S subunit of the ribosome.
Is kanamycin used in neospora caninum tacyzoite culture?
No
Name a medicine that starts with the letter k?
Kanamycin Sulfate is all I can think of...
How do you identify bacterial cells that have taken up your gene of interest?
A standard test is the antibiotic resistance take up. This is where you confer resistance plasmids containing the gene of interest to bacterial uptake. So you use two antibiotics, Kanamycin and Penicillin, to test that your bacteria so infused is now resistant to these two antibacterial agents. A successful recombination exercise will lead to growth on this medium.
What is kantrex made up of?
Kantrex contains kanamycin, which is an aminoglycoside antibiotic, and thus acts on the 30S subunit of ribosomal RNA. It is similar to gentamycin.
How will you identify cells carrying the recombinant plasmid?
The transformants are selected for on agar containing an appropriate antibiotic. For example if your recombinant plasmid contains a kanamycin cassette, then only the cells containing the plasmid will grow on an agar plate containing kanamycin. PCR can then be performed on the colonies to ensure they contain your gene of interest on the plasmid.
