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EMB is an undefined selective/differential medium. It contains aniline dyes (methylene blue and eosin), which inhibit the growth of Gram-positive bacteria selecting for Gram-negative bacteria. EMB also contains lactose which makes the media differential based on an organisms ability to ferment lactose
This type of agar is designed to grow gram-negative bacteria. It will not grow gram-positive bacteria due to a dye in the formula of the agar.
It is desirable because it is easier to prepare.
Mannitol salt agar or MSA is a commonly used growth medium in microbiology. It contains a high concentration (~7.5%-10%) of salt (NaCl), making it selective for Staphylococci (and Micrococcaceae) since this level of NaCl is inhibitory to most other bacteria.
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Leboffe & Pierce in their text "Microbiology Laboratory Theory and Application" (second edition) state on page 111 that PEA is an undefined medium
Undefined.
A term that is undefined mean simply that it is not defined. The prefix un means not, and if you add it to the word defined, ta da, you get undefined.
"Defined items" are defined in terms of "undefined terms".
they are not defined
A set can be defined or undefined.
"Defined items" are defined in terms of "undefined terms".
It's complex because the constituent elements of the medium (enzymatic digests of casein and soybean) are present in an undefined amount, and an amount which will vary from batch to batch. Contrast this to a chemically defined medium which will always contain the exact amount of constituent.
Zero is pretty well defined. Division by zero is undefined.
EMB is an undefined selective/differential medium. It contains aniline dyes (methylene blue and eosin), which inhibit the growth of Gram-positive bacteria selecting for Gram-negative bacteria. EMB also contains lactose which makes the media differential based on an organisms ability to ferment lactose
There are two main classifications of culture medianamely defined and undefined. An undefined culture mediumcontains a basic source of aminoacids and nitrogen which are the essential requirements for growth of microorganisms. Common examples of the constituents are beef and yeast extracts. The exact composition and percentage of the individual amino acid or nitrogen providing source is unknown and this is used for basic culture and maintenance of strains. An undefined medium is also called a basal or complex medium. In addition to the aminoacid and nitrogen source it also contains a source for glucose, water and other essential salts.A defined culture medium or synthetic medium is one that contains salts and a glucose source and other components based on the requirements of culture. It contains no yeast or animal extracts. Each of the components used are accurately weighed and the percentage known.