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What is Loss of heterozygosity?
LOH stands for Loss of Heterozygosity. LOH happens when a somatic cell consist only of one copy of allele caused by non-disjunction in mitosis, segregation all through out re-combination, or during chromosome segment deletion.
What is the difference between homozygosity and heterozygosity?
Homozygosity is the condition of having two identical alleles from the parents for a gene (AA). Heterozygosity is the condition of having two different alleles for a trait from parents (Aa).
Human Karyotype-Nomenclature Human genome?
To determine the commonly deleted region on chromosome 3 in carcinoma of the uterine cervix, these samples were also examined for loss of heterozygosity at 5 other loci on chro mosome 3: RAFl(3p24-25) (14); ERBAß(3p22-24.l ) (17); DNF15S2 (3p21) (13); D3S3(3pl4) (13); and SST(3q28) (13). Loss of heterozygosity at the RAF1 locus and the ERBAßlocus was observed in one of 3 patients and 3 of 7 patients (Fig. 1, b c), respectively, but no loss was observed at 2 other loci, DNFI5S2 and SST, whereas duplication of one of two alÃeles was observed in 2 of 6 patients at SST (Table 1). Analysis of loss of heterozygosity at the ERBAßlocus was performed using 2 different DNA probes: a human ERBAßcomplementary DNA clone, pheA4; and a genomic ERBAßDNA clone, pBH302 (15- 17). The pheA4 and pBH302 probes detect BamHl and Hindlll RFLP, respectively, and loss of heterozygosity at this locus was observed in none of 2 patients by using the pheA4 probe and in 3 of 5 patients by the pBH302 probe (Table 1; Fig. lé).No information was available on loss of heterozygosity at the D3S3 locus, because none of the 18 patients was heterozygous in normal tissue at this locus. Therefore, the commonly deleted chromosomal region in carcinoma of the uterine cervix is probably a small part of the short arm of chromosome 3, including the D3S2 locus. These results strongly suggest that recessive genetic changes on chromosome 3p are involved in the development of carcinoma of the uterine cervix.
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