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What else can I help you with?
What part of speech is the word cassette?
Cassette is a noun.
If you place gene in reverse orientation how can it be detected?
Most genes contain restrictions sites. Once you've inserted your gene into the vector you use restrictions sites in the gene and the vector to cut the vector into smaller pieces. If the pieces correspond to the pattern you expect for a reverse orientated gene then you know it is in the reverse orientation.
How do you predict a PCR product size from a gene cloned into an expression vector?
It all depends on where you primers are. Presumably you will have one primer that sits on the cloned gene and one that sits on the vector (that way you only get a product if the gene has cloned successfully). As long as you know where your primers land, it should be easy to work out how big the PCR product will be simply by adding the distance from the primer on the gene to the end of the gene and the distance from the primer on the vector to the end of the vector.
What is a positive selection vector?
A positive selection vector is a type of vector used in molecular biology that contains a gene conferring a specific trait or resistance to a selection agent, such as an antibiotic. This allows for the selection of only those cells that have successfully taken up the vector and integrated the gene.
How a gene for making pyrethrins is transferred from daisy to yeast?
The gene for making pyrethrins can be transferred from a daisy to yeast using recombinant DNA technology. This involves isolating the gene from the daisy, inserting it into a plasmid vector, and then introducing the vector into yeast cells. The yeast cells can then use the gene to produce pyrethrins as part of their normal metabolic processes.
Where is the first place scientists look to insert a new gene?
Scientists often look to insert a new gene into a vector, such as a plasmid or a viral vector. Vectors are vehicles that can deliver the gene into a host organism's cells for expression and study.
Why is cloning an example of cloning?
Gene Cloning is used to clone a gene of interest in a vector called plasmid. The chimeric DNA or rDNA formed by cloning is stable and can be used to propagate and sequence the DNA. producing vector containing inulin gene is an example.
Gene cloning can involve inserting genes into?
Gene cloning involves inserting a gene of interest into a plasmid or a vector that can replicate inside a host cell. The plasmid or vector is then introduced into a host cell where the gene can be replicated along with the host cell's own DNA. This allows researchers to produce large quantities of the gene of interest for further study or applications.
Direction of vector in space is specified by?
It is an integral part of the vector and so is specified by the vector.
What is the role of Cloning Vector in recombinant DNA technology?
A cloning vector is a DNA molecule used to carry a foreign DNA fragment into a host cell for replication. It serves as a vehicle for the insertion of DNA fragments and allows for the propagation of recombinant DNA. Cloning vectors typically contain sequences for replication, selection, and insertion of foreign DNA.
In the process of human gene cloning using recombinant plasmids what is the bacterial plasmid?
The bacterial plasmid is a small circular DNA molecule that is used as a vector to carry the gene of interest in gene cloning experiments. It is introduced into bacteria, where it replicates independently from the bacterial chromosome. The gene of interest is inserted into the plasmid using restriction enzymes and ligase.
What is a vector of magnitude?
Nothing. A magnitude is part of a vector. For example, for the vector "10 metres due East", 10 metres is the magnitude of the vector and East is the direction of the vector.
