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What factors affect enzyme activity?

Updated: 8/10/2023
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11y ago

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Temperature - too cold the enzyme will still work but slowly, too hot and the enzyme will become denatured . As temperature increases, the kinetic energy of the molecules increases so they move around more, meaning that there are more collisions between the enzymes and substrate molecules and therefore more reactions.

pH - different types of enzymes work best in different pH environments. A change in pH interferes with the shape of the enzymes active site (where it bonds and reacts with substrate) and therefore does not fit the shape of the substrate as well so the enzyme is unable to work on the substrate.

enzyme and substrate concentration - how many there is of each. Changing the concentrations of enzyme and substrate concentrations will affect the number of collisions between them and therefore the number of reactions.

enzyme inhibitors - these are molecules which bind to enzymes, reducing their activity (many drugs are enzyme inhibitors).

co-factors - these are chemical compounds which bind to enzymes and which are needed by the enzyme to work on substrate molecules. They are often called helper molecules.

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11y ago
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12y ago

temperature- enzymes work best at a temperature of 37 C (body temp)

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10y ago

temperature, pH, substrate concentration, and the presence or absence of inhibitors.

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Q: What factors affect enzyme activity?
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