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phase. I think
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Why did Hershey and Chase grow viruses in cultures that contained both radioactive Sulfur. What might have happened if they had used only one radioactive substance.?
Hershey and Chase used radioactive Sulfur to label viral proteins, as proteins contain sulfur. By growing the viruses in a culture containing both radioactive Sulfur and Phosphorus, they could differentiate between viral proteins (labeled with Sulfur) and viral DNA (labeled with Phosphorus). If they had only used one radioactive substance, they would not have been able to determine the specific molecule (protein or DNA) that the virus injected into the host cell.
Why did Hersheys and chase grow viruses in cultures that contained both radioactive phosphorus and radioactive sulfur?
Phosphorous 32 was used to label the Nucleic Acid. Sulfur 35 was used to label the protein. The progeny of the E. coli cells Hershey and Chase grew the virus in had Phosphorous 32 in them which concluded that the DNA went inside and was the genetic material. The phage coating left on the outside tested positive for Sulfur 35 which meant protein was left on the outside.
What reaction is used to radioactivley label DNA?
The reaction used to radioactively label DNA is the random primer labeling reaction. This involves using short oligonucleotide primers that hybridize randomly to the DNA template, which are then extended using a DNA polymerase in the presence of radioactive nucleotides.
What did hershey and chase discover after the p-labeled phages infected the bacteria most of the radioactive phosphorous found in the layer containing?
Hershey and Chase discovered that after the P-labeled phages infected the bacteria, most of the radioactive phosphorus was found in the viral DNA, while little to no radioactive sulfur (used to label protein) was found. This confirmed that genetic material, not protein, is responsible for heredity in viruses.
What of the three experiments gave evidence that protein was not the genetic material?
The experiment by Alfred Hershey and Martha Chase in 1952 provided evidence that protein was not the genetic material. They used bacteriophages, which are viruses that infect bacteria, and labeled the DNA with radioactive phosphorus and the protein coat with radioactive sulfur. After allowing the viruses to infect bacteria, they found that only the radioactive phosphorus (DNA) entered the bacterial cells, while the radioactive sulfur (protein) remained outside. This demonstrated that DNA, not protein, was the hereditary material responsible for passing genetic information.
Why is radioactive sulfur used?
Radioactive sulfur is used in scientific research to trace the pathway of sulfur in biological systems and to understand sulfur metabolism in different organisms. It can help researchers study processes like protein synthesis, enzyme activity, and cell division.
What has an isotope mass of 32 and is used to label proteins?
sulfur
Why did the scientists use radioactive sulfur and phosphorus in this experiment?
Phosphorous 32 was used to label the Nucleic Acid. Sulfur 35 was used to label the protein. The progeny of the E. coli cells Hershey and Chase grew the virus in had Phosphorous 32 in them which concluded that the DNA went inside and was the genetic material. The phage coating left on the outside tested positive for Sulfur 35 which meant protein was left on the outside.
Where was the radioactive sulfur found at the end of experiment one in the hershey-chase experiment?
At the end of Experiment One in the Hershey-Chase experiment, radioactive sulfur, which labeled the protein coats of the T2 bacteriophage, was found in the supernatant, indicating that the protein did not enter the bacterial cells. This suggested that proteins were not the genetic material responsible for transferring traits to the bacteria. In contrast, the radioactive phosphorus used to label DNA was found in the bacterial cells, supporting the conclusion that DNA is the genetic material.
What is the Radioactive molecule used to label proteins?
Oh, dude, it's like so simple. The radioactive molecule used to label proteins is typically 35S (sulfur-35) or 32P (phosphorus-32). They're like the rockstars of protein labeling, shining brightly in the world of molecular biology. Just remember, handle with care - you don't want to glow in the dark accidentally.
What reaction is used to radioactively label DNA?
The reaction used to radioactively label DNA is typically performed using a DNA polymerase enzyme along with radioactive nucleotides, such as [α-32P]dNTPs. This allows for the incorporation of the radioactive label into the DNA strand during the polymerase reaction.
What is radioactive yellow II label?
The radioactive yellow II label is a symbol used to indicate materials that are radioactive and pose a health hazard. It is typically a yellow triangle containing the radiation symbol and is used to warn individuals of potential exposure to radiation in the area. It is important to follow safety protocols and handle these materials with care.
