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An enzyme that cuts double-stranded DNA at specific nucleotide sequences?
Such an enzyme is called a restriction endonuclease
Why you add distilled water in restriction digestion of lambda DNA along with enzyme and assay buffer?
We generally add milliQ (DNase free) water. For digestion water is added usually. It provides right Hydrogen bonds between enzyme amino acid residues and bases present in the restriction sites. Utpal Roy
What is The role of buffer in restriction digestion reactions?
I believe that it is used to stabilize the pH levels so the enzyme activity isn't effected.
What are the principles of restriction enzyme use in removing sections of the genome?
Restriction enzymes can act only on double strand DNA . Restriction enzyme recognizes and hydrolyzes the backbone of DNA between deoxyribose and phosphate groups at or near the restriction sites. This leaves a phosphate group on the 5` ends and a hydroxyl on the 3` end of both the strands . Thus digestion with restriction enzymes results in the fragmentation of the double stranded DNA molecule.
What is the restriction site of the restriction enzyme TaqI?
TaqI's restriction site is:TCGAAGCT
Hi does any one know if I want to put some restriction enzyme close to cDNA should I put some nucleotide befor this restriction enzyme?
Restriction enzyme cuts DNA strand at specific locations Restriction enzyme cuts DNA strand at specific locations
What is the role of BSA in restriction enzyme activity?
BSA is used to stabilize some enzymes during digestion of DNA and to prevent adhesion of the enzyme to reaction tubes and other vessels. This protein does not affect other enzymes that do not need it for stabilization.
How do you identify restriction enzyme?
By playing with you.
Is topoisomerases belong to restriction enzymes?
Topoisomerase is not a restriction enzyme but an enzyme that keeps unwound DNA from tangling while it is being replicated.
Why restriction enzyme cannot cut its own DNA?
Restriction enzymes are produced by bacteria to help destroy foreign, invading DNA, such as the DNA of bacteriophage (a virus that infects bacterial cells). Every restriction enzyme comes with a methylase enzyme, or more specifically, a DNA methyltransferase. The methylase enzyme methylates (adds a methyl group) to the restriction endonuclease site on the cell's own DNA, which protects the sites from the restriction enzyme so that it does not degrade its own DNA.
Why did you cut both segments of DNA with the same restriction enzyme?
You use the same enzyme inn order to get the same restriction and binding sites.
What enzyme is responsible for fat digestion?
the lipase enzyme :)
