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Topoisomerase is not a restriction enzyme but an enzyme that keeps unwound DNA from tangling while it is being replicated.
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Which pair of enzymes is necessary to make recombinant DNA?
Actually the answer would be Restriction enzyme and DNA ligase.
Enzymes used to cut DNA molecules in recombinant DNA researsh are?
restriction enzymes
How are large DNA molecules cut up?
by using restriction enzymes
What are enzymes cutting DNA at specific sites to form restriction fragments called?
restriction enzymes or endonuclease enzymes
What is used to cut the DNA chain so that new genes may be inserted?
Restriction Enzymes
What enzyme do scientists use to cut genes out of strands if DNA?
restriction enzymes
Which pair of enzymes is necessary to make recombinant DNA?
Actually the answer would be Restriction enzyme and DNA ligase.
Enzymes used to cut DNA molecules in recombinant DNA researsh are?
restriction enzymes
Do restriction enzymes cut protein molecules at specific sites?
No. restriction enzymes do not cut proteins. restriction enzymes cut DNA molecules at specific sites called restriction sites.
How are large DNA molecules cut up?
by using restriction enzymes
What are enzymes cutting DNA at specific sites to form restriction fragments called?
restriction enzymes or endonuclease enzymes
Do eukaryotic cells have restriction endonuclease?
Restriction enzymes are a class of enzymes called endonucleases. Endonucleases are able to cut in the middle of the DNA backbone or the phosphodiester bonds. A different class of enzymes called exonucleases cut the DNA backbone, but only from the ends - either from the 3' end or the 5' end. MOST restriction endonucleases are prokaryotic in origin. However, there are several found in eukaryotic cells, including our own. In eukaryotes they are not referred to as restriction enzymes, just endonucleases. An example of an endonuclease in eukaryotes is Apn1, isolated from yeast. This enzyme helps prevent DNA damage from environmental agents. Another common enzyme family called the topoisomerases (DNA Gyrase) has endonuclease activity. Topoisomerases prevent the supercoiling of DNA at replication forks, by cutting the backbone, relieving the tension and pasting the ends together again - hence the endonuclease activity. In prokaryotes, restriction enzymes actually restrict the proliferation of viruses by cleaving their nucleic acids at specific base-pair sequences. These enzymes cut DNA at the exact same sequence no matter which organism the DNA belongs to - that's why they're such powerful tools in genetic engineering. Eukaryotic endonucleases may not all help in restricting invading nucleic acids and in fact perform many distinct "jobs". That is probably why they are never referred to as restriction enzymes.
What is used to cut the DNA chain so that new genes may be inserted?
Restriction Enzymes
Which of the following cuts DNA molecules at specific locations?
restriction endonuclease enzymes
What organism uses restriction enzymes?
Bacterias use restriction enzymes as a form of defense mechanism. We as people use these restriction enzymes in bacterias to aid us in genetic engineering.
What does a geneticist use to cut DNA at specific base sequences?
Restriction enzymes, also known as restriction endonucleases, are used to cut DNA into smaller fragments. Restriction enzymes are found in bacteria, where they act like molecular scissors by cutting up DNA from invading viruses or bacteriophages. Each restriction enzyme recognizes a specific nucleotide sequence and cuts the DNA at that site. This process makes restriction enzymes extremely useful in biotechnology where they are used in procedures such as DNA cloning, DNA fingerprinting, and genetic engineering. There are hundreds of known restriction enzymes, and each one was named for the bacteria from which it was isolated. For example, EcoRI was isolated from Escherichia coli and HaeIII from Haemophilus aegyptius.
Are bacteria a source of restriction enzymes in DNA?
Restriction enzymes are made by the ribosomes in bacteria. For information on which bacteria produce which specific restriction enzymes, some can be found in a table near the bottom of this page: http://en.wikipedia.org/wiki/Restriction_enzymes
