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Purification of the DNA sample. Phenol is carbolic acid and is used to dissolve proteins and other cellular debris associated with the DNA being prepared for analysis.
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What is role of potassum cloride in Tkm1 buffer for DNA isolation?
for cell lysis
What is the role of sorbitol in DNA isolation?
what is the function of sorbitol in producing plant protoplasts
Function of phenol DNA extraction?
phenol helps to remove non polar proteins and lipids from the solution
What is the function of phenol in DNA extraction?
phenol helps to remove non polar proteins and lipids from the solution
What is the principle involved in the isolation procedure?
DNA isolation is a based on the principle of purification. DNA samples are isolated through the use of physical and chemical methods. Friedrich Miescher conducted the first isolation of DNA in 1869.
Role of phenol chloroform in DNA isolation?
phenol is used in order to remove protein impurities from the DNA to yield pure dna while chloroform prevents shearing of DNA during isolation.
Role of phenol chloroform isoamyl alcohol in plasmid DNA extraction?
The actual role of phenol chloroform isoamyl alcohol in a plasmid DNA extraction is to purify the DNA. The alcohol will act in part as a detergent.
Role of sucrose in DNA isolation from human blood?
Sucrose performs the function of osmoregulation in the protocol of DNA isolation from blood
What is role of Cetyl trimethyl ammonium bromide in DNA isolation?
it act as as a cationic detergent for the isolation dna from the given sample
What is the role of beta-mercaptoethanol in DNA extraction?
stabilization of phenol against oxidation
Role of chloroform in DNA isolation?
It solubilizes lipids and a lot of proteins to remove them from the DNA.
Role of CTAB in DNA isolation?
It sequester carbohydrates in the solution
What is the role of SE-VEG in DNA isolation?
the role seveg in plant DNA extractions is to remove chlorophyll and similar pigments
Function chloroform in extraction DNA from blood?
to remove excess phenol from DNA to remove excess phenol from DNA
What is role of potassum cloride in Tkm1 buffer for DNA isolation?
for cell lysis
What is the role of 2-mercaptoethanol in the isolation of genomic DNA from a plant source?
It is an antioxidant.
What is the role of sorbitol in DNA isolation?
what is the function of sorbitol in producing plant protoplasts
