what is the function of sorbitol in producing plant protoplasts
it is a buffer.
for cell lysis
Purification of the DNA sample. Phenol is carbolic acid and is used to dissolve proteins and other cellular debris associated with the DNA being prepared for analysis.
DNA isolation is a based on the principle of purification. DNA samples are isolated through the use of physical and chemical methods. Friedrich Miescher conducted the first isolation of DNA in 1869.
it iS A ionic detergent disrupts bonds between lipids and fosters the isolation of DNA....
70% ethnol is used for the dna isolation becuse it makes hydrogen bonding with the water molecules and makes DNA hydrophopic so that it pricipitated.
Sucrose performs the function of osmoregulation in the protocol of DNA isolation from blood
it act as as a cationic detergent for the isolation dna from the given sample
It solubilizes lipids and a lot of proteins to remove them from the DNA.
It sequester carbohydrates in the solution
the role seveg in plant DNA extractions is to remove chlorophyll and similar pigments
phenol is used in order to remove protein impurities from the DNA to yield pure dna while chloroform prevents shearing of DNA during isolation.
for cell lysis
It is an antioxidant.
sodiom acetat reaction with membrane protein and cause that persipitate and help to dna isolation
spooling of the DNA results from the addition of the ethanol which is insoluble in the solution. after we get he DNA in the form of spooling structure the solution is centrifuged. so we get the DNA.
mgcl2 work as a cofactor and they enhance the enzymatic reaction..
beta- merceptoethanol denatures the protein by breaking the sulphur bridges in it.